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OPAC HELP

Titolo:
ROLE OF LIPIDS IN THE PERMEABILIZATION OF MEMBRANES BY CLASS-L AMPHIPATHIC HELICAL PEPTIDES
Autore:
POLOZOV IV; POLOZOVA AI; TYTLER EM; ANANTHARAMAIAH GM; SEGREST JP; WOOLLEY GA; EPAND RM;
Indirizzi:
MCMASTER UNIV,HLTH SCI CTR,DEPT BIOCHEM,1200 MAIN ST W HAMILTON ON L8N 3Z5 CANADA MCMASTER UNIV,HLTH SCI CTR,DEPT BIOCHEM HAMILTON ON L8N 3Z5 CANADA UNIV ALABAMA,MED CTR,DEPT MED BIRMINGHAM AL 35294 UNIV ALABAMA,MED CTR,DEPT BIOCHEM BIRMINGHAM AL 35294 UNIV ALABAMA,MED CTR,ATHEROSCLEROSIS RES UNIT BIRMINGHAM AL 35294 UNIV TORONTO,DEPT CHEM TORONTO ON M5S 1A1 CANADA
Titolo Testata:
Biochemistry
fascicolo: 30, volume: 36, anno: 1997,
pagine: 9237 - 9245
SICI:
0006-2960(1997)36:30<9237:ROLITP>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOSPHOLIPID-BILAYERS; BIOLOGICAL-MEMBRANES; MARKER MOLECULES; VESICLE CONTENTS; ION CHANNELS; LIPOSOMES; KINETICS; FUSION; MAGAININ-2; MECHANISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
I.V. Polozov et al., "ROLE OF LIPIDS IN THE PERMEABILIZATION OF MEMBRANES BY CLASS-L AMPHIPATHIC HELICAL PEPTIDES", Biochemistry, 36(30), 1997, pp. 9237-9245

Abstract

We studied the mechanism of membrane permeabilization by the 18L model peptide (GIKKFLGSIWKFIKAFVG), which features the consensus class L sequence averaged from the number of naturally occurring lytic peptides. Two aspects of membrane lipid composition significantly affected peptide-membrane interactions: the presence of acidic Lipids and, in zwitterionic membranes, and the presence of nonbilayer forming lipids. In zwitterionic membranes, 18L peptide destabilizes the membrane, leadingto a transient formation of large defects in the membrane which result generally in contents leakage, but in the presence of bilayer-bilayer contact can alternatively lead to vesicle fusion. In membranes containing acidic lipids (DOPC:DOPG, DOPG), 18L caused leakage but not fusion, probably due to mutual repulsion of acidic vesicles. While the extent of contents leakage was approximately the same as for zwitterionicmembranes, the kinetics of leakage could be resolved only by using stopped-flow, leakage being essentially complete within the first minute. Previously, we reported that apolipoprotein (class A) and lytic (class L) peptide analogs have opposing effects on some properties of biological membranes. This reciprocal effect of 18L and Ac-18A-NH2, class A model peptide, is restricted to membranes with a high propensity fornonbilayer phase formation (DOPE, Me-DOPE, DOPC:DOPE, DOPC:Me-DOPE). The decrease in the content of nonbilayer phase forming lipid or the addition of acidic lipids reduces or eliminates the reciprocal effects. This suggests the importance of nonbilayer phase propensity for certain functions of biological membranes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 23:48:48