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Titolo:
Characterization of human villous and extravillous trophoblasts isolated from first trimester placenta
Autore:
Tarrade, A; Kuen, RL; Malassine, A; Tricottet, V; Blain, P; Vidaud, M; Evain-Brion, D;
Indirizzi:
Univ Paris 05, Fac Sci Pharmaceut & Biol Paris, INSERM, U427, F-75270 Paris 06, France Univ Paris 05 Paris France 06 is, INSERM, U427, F-75270 Paris 06, France Univ Paris 05, Fac Sci Pharmaceut & Biol Paris, Lab Microscopie Elect, F-75270 Paris, France Univ Paris 05 Paris France F-75270 roscopie Elect, F-75270 Paris, France Univ Paris 05, Fac Sci Pharmaceut & Biol Paris, Genet Mol Lab, F-75270 Paris 06, France Univ Paris 05 Paris France 06 s, Genet Mol Lab, F-75270 Paris 06, France Hop Robert Debre, Lab Foetopathol, F-75019 Paris, France Hop Robert DebreParis France F-75019 Foetopathol, F-75019 Paris, France
Titolo Testata:
LABORATORY INVESTIGATION
fascicolo: 9, volume: 81, anno: 2001,
pagine: 1199 - 1211
SICI:
0023-6837(200109)81:9<1199:COHVAE>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; GAP JUNCTIONAL COMMUNICATION; IN-VITRO; CYTOTROPHOBLAST DIFFERENTIATION; GENE-EXPRESSION; IMMUNOHISTOCHEMICAL LOCALIZATION; CHORIONIC-GONADOTROPIN; ENDOVASCULAR INVASION; EXTRACELLULAR-MATRIX; MESSENGER-RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Evain-Brion, D Univ Paris 05, Fac Sci Pharmaceut & Biol Paris, INSERM, U427, 4 Ave Observ, F-75270 Paris 06, France Univ Paris 05 4 Ave Observ ParisFrance 06 aris 06, France
Citazione:
A. Tarrade et al., "Characterization of human villous and extravillous trophoblasts isolated from first trimester placenta", LAB INV, 81(9), 2001, pp. 1199-1211

Abstract

Trophoblasts of the human placenta differentiate along two pathways to give either extravillous cytotrophoblasts (EVCT) with invasive properties and that are implicated in the implantation process, or villous cytotrophoblasts (VCT) that by cell fusion form multinucleated syncytiotrophoblasts. We report the first isolation and purification of these two cell types from the same chorionic villi of first trimester human placenta. We also studied their differentiation in vitro. Electron microscopy showed that in contrast toVCT, EVCT had no microvilli but contained large fibrinoid inclusions. EVCTcultures required a matrix to invade, and as previously established, VCT cultured on plastic dishes aggregated and fused to form syncytiotrophoblasts. These differentiation processes were characterized by a particular pattern of gene expression as assessed by real-time PCR and confirmed by immunocytochemical analysis of the corresponding proteins. EVCT cultured in vitro expressed high levels of HLA-G, c-erbB2, human placental lactogen, and very little human chorionic gonadotropin. Interestingly, TGF beta2 was a marker of EVCT in vitro and in situ. These data offer a new tool for cell biologists to study the molecular mechanisms involved in human placental development and its pathology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:10:33