Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Anti-B7-1 blocks mononuclear cell adherence in vasa recta after ischemia
Autore:
De Greef, KE; Ysebaert, DK; Dauwe, S; Persy, V; Vercauteren, SR; Mey, D; De Broe, ME;
Indirizzi:
Univ Antwerp, Dept Expt Surg, B-2020 Antwerp, Belgium Univ Antwerp Antwerp Belgium B-2020 t Expt Surg, B-2020 Antwerp, Belgium Univ Antwerp, Dept Nephrol, B-2020 Antwerp, Belgium Univ Antwerp AntwerpBelgium B-2020 ept Nephrol, B-2020 Antwerp, Belgium
Titolo Testata:
KIDNEY INTERNATIONAL
fascicolo: 4, volume: 60, anno: 2001,
pagine: 1415 - 1427
SICI:
0085-2538(200110)60:4<1415:ABMCAI>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE-RENAL-FAILURE; EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; COLD ISCHEMIA/REPERFUSION INJURY; REPERFUSION INJURY; CD28-B7 BLOCKADE; ENDOTHELIAL-CELL; ICAM-1; RAT; ANTIBODIES; PREVENTS;
Keywords:
congestion; costimulatory pathway; ischemia-reperfusion; vasa recta; T cell; renoprotection; cell signaling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: De Broe, ME Univ Antwerp Hosp, Dept Nephrol Hypertens, Wilrijkstr 10, B-2650 Edegem, Belgium Univ Antwerp Hosp Wilrijkstr 10 Edegem Belgium B-2650 Belgium
Citazione:
K.E. De Greef et al., "Anti-B7-1 blocks mononuclear cell adherence in vasa recta after ischemia", KIDNEY INT, 60(4), 2001, pp. 1415-1427

Abstract

Background. Blocking the costimulatory pathway by CTLA-4 Ig, reactive withboth B7-1 and B7-2 costimulatory molecules, protects the kidney during acute ischemia/reperfusion injury. This study investigated whether and how B7-1 and/or B7-2 proteins are involved in renal ischemia/reperfusion injury (IRI). Methods. Uninephrectomized rats were submitted to warm renal ischemia (30 min) and received control monoclonal antibody (mAb: 17E3), anti-B7-1 (3H5).anti-B7-2 (24F), a combination of anti-B7-1/B7-2, or CTLA-4 Ig. Renal function, morphology, and the kinetics of inflammatory cells were studied for aten-day period, Binding sites of the injected antibodies were detected by secondary staining with anti-mouse Ab. Results. Compared with controls, acute renal failure (ARF) in the anti-B7-1 group was attenuated both functionally and morphologically. Anti-B7-1/B7-2 and CTLA-4 Ig also were protective in IRI. ARF was not altered by anti-B7-2 treatment. Two hours after reperfusion, B7-1 was expressed along the endothelial cells of the ascending vasa recta. Expression of B7-1 increased over time during the first 24 hours and decreased thereafter. Two hours afterreperfusion, adherence/accumulation of T cells and monocytes/macrophages was found in the vasa recta of the ischemic kidney. Anti-B7-1-treated animals had fewer T cells and monocytes/macrophages in the vasa recta compared with controls. Leukocyte accumulation in these vessels after anti-B7-2 treatment was not different from IRI controls,Conclusion. These observations strongly support the key role of the B7-1 protein in the protection of renal IRI through inhibition of T cell and monocyte adherence at the level of the ascending vasa recta.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 19:14:52