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Titolo:
Ganglioside as an endogenous growth suppressor for glomerular mesangial cells
Autore:
Tsuboi, N; Utsunomiya, Y; Kawamura, T; Kawano, T; Hosoya, T; Ohno, T; Yamada, H;
Indirizzi:
Jikei Univ, Sch Med, Inst DNA Med, Dept Mol Genet,Minato Ku, Tokyo 105, Japan Jikei Univ Tokyo Japan 105 d, Dept Mol Genet,Minato Ku, Tokyo 105, Japan Jikei Univ, Sch Med, Dept Internal Med, Div Kidney & Hypertens, Tokyo 105,Japan Jikei Univ Tokyo Japan 105 Med, Div Kidney & Hypertens, Tokyo 105,Japan
Titolo Testata:
KIDNEY INTERNATIONAL
fascicolo: 4, volume: 60, anno: 2001,
pagine: 1378 - 1385
SICI:
0085-2538(200110)60:4<1378:GAAEGS>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
PUROMYCIN NEPHROSIS; TUMOR GANGLIOSIDES; IMMUNE-RESPONSE; T-CELLS; RAT; PROLIFERATION; INHIBIT; GLYCOSPHINGOLIPIDS; DIFFERENTIATION; MODULATION;
Keywords:
cell proliferation; hypercellularity; renal injury; sialylated glycosphingolipids; anti-inflammatory; mesangioproliferative glomerulonephritis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Tsuboi, N Jikei Univ, Sch Med, Inst DNA Med, Dept Mol Genet,Minato Ku, 3-25-8 Nishi Shinbashi, Tokyo 105, Japan Jikei Univ 3-25-8 Nishi Shinbashi Tokyo Japan 105 yo 105, Japan
Citazione:
N. Tsuboi et al., "Ganglioside as an endogenous growth suppressor for glomerular mesangial cells", KIDNEY INT, 60(4), 2001, pp. 1378-1385

Abstract

Background. Glomerular mesangial cells potentially secrete many grow th-modula ting substances that could regulate mesangial cell proliferation. To date, however, the properties of such factors have not been fully evaluated. Methods. For that purpose, conditioned medium (CM) from mesangial cells was used for cross-feeding experiments. Cell proliferation was evaluated by H-3-thymidine incorporation assay and direct cell counting. The growth-regulatory molecule was further characterized using biochemical techniques. Results. Cross-feeding this CM to mesangial cells in vitro. despite stimulation with platelet-derived growth factor (PDGF). effectively suppressed the cells' synthesis of DNA in a dose-dependent manner. The inhibitory substance derived from mesangial cells was less than 3 kD in molecular mass. was heat stable, and was insensitive to proteinase K. After neuraminidase digestion. this inhibitory activity was lost. These data indicated that the inhibiting substance bore the typical features of gangliosides, which are multifunctional glycolipids that reside in cell membrane. Gangliosides were abundant in the CM from mesangial cells. as detected by metabolic radiolabelingand thin-laver chromatography (TLC). This result suggested that mesangial cells constitutively shed gangliosides. The growth suppressive activity in the CM was blunted when mesangial cells were pretreated with the ganglioside synthesis inhibitor d-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol-HCl (d-threo-PDMP: 20 mu mol/L) in accordance with the decreased ganglioside content in cells. Finally. gangliosides isolated from CM of mesangialcells suppressed PDGF-induced DNA synthesis of mesangial cells. Conclusions. These results suggest that mesangial cells constitutively shed gangliosides that then suppress the division of these cells in an autocrine-like manner.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 21:07:55