Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Modulation of endothelin-1-induced cytosolic free calcium mobilization andmitogen-activated protein kinase activation by erythropoietin in vascular smooth muscle cells
Autore:
Kusano, E; Akimoto, T; Umino, T; Yanagiba, S; Inoue, M; Ito, C; Ando, Y; Asano, Y;
Indirizzi:
Jichi Med Sch, Dept Nephrol, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan
Titolo Testata:
KIDNEY & BLOOD PRESSURE RESEARCH
fascicolo: 3, volume: 24, anno: 2001,
pagine: 192 - 200
SICI:
1420-4096(2001)24:3<192:MOECFC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT-HUMAN-ERYTHROPOIETIN; SPONTANEOUSLY HYPERTENSIVE RATS; SIGNAL-TRANSDUCTION; ENDOTHELIAL-CELLS; DNA-SYNTHESIS; CA2+; STIMULATION; EXPRESSION; RESPONSES; INCREASE;
Keywords:
erythropoietin; endothelin-1; cytosolic free Ca2+ mobilization; MAP kinase; vascular smooth muscle cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Kusano, E Jichi Med Sch, Dept Nephrol, Yakushiji 3311-1, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Yakushiji 3311-1 Minami Kawachi TochigiJapan 3290498
Citazione:
E. Kusano et al., "Modulation of endothelin-1-induced cytosolic free calcium mobilization andmitogen-activated protein kinase activation by erythropoietin in vascular smooth muscle cells", KIDNEY BL P, 24(3), 2001, pp. 192-200

Abstract

Background. It has been reported that human recombinant erythropoietin (rHuEPO) modulates the sensitivity of the cardiovascular system to vasoconstrictors. We investigated whether rHuEPO has modulative effects on the endothelin-1 (ET-1)-induced elevation of cytosolic free calcium concentration ([Ca2+](i)) and mitogen-activated protein (MAP) kinase activation in vascular smooth muscle cells (VSMC). Methods: [Ca2+](i) was measured by fura-2/AM, and MAP kinase activation was analyzed by Western blotting. Results: Exposureof VSIVIC to rHuEPO prior to stimulation with ET-1 enhanced both basal andET-1-induced elevation of [Ca2+](i) in a dose-dependent manner in the presence of extracellular Ca2+. The synergistic effect was also retained in theabsence of extracellular Ca2+ after exposure to rHuEPO. However, the effect was diminished in the presence of extracellular Ca2+ combined with the intracellular Ca2+ release inhibitor TMB-8, PKC inhibitor, or PKC depletion. Exposure to rHuEPO also had a synergistic effect on the activation of MAP kinase induced by ET-1; however, this effect was diminished in the presence of the Ca2+ chelator BAPTA-AM. Conclusion: The results suggest that rHuEPO has synergistic effects on ET-1-induced [Ca2+], mobilization, particularly on intracellular Ca2+ release, and MAP kinase activation in VSMC. Copyright(C) 2001 S. Karger AG, Basal.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 20:48:42