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Titolo:
Hippurate metabolism as a hydroxyl radical trapping mechanism in the rat kidney
Autore:
Malyusz, M; Kahler, W; Gronow, G;
Indirizzi:
Univ Kiel, Inst Physiol, D-24118 Kiel, Germany Univ Kiel Kiel Germany D-24118 Kiel, Inst Physiol, D-24118 Kiel, Germany
Titolo Testata:
KIDNEY & BLOOD PRESSURE RESEARCH
fascicolo: 3, volume: 24, anno: 2001,
pagine: 149 - 158
SICI:
1420-4096(2001)24:3<149:HMAAHR>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL INJURY; CYTOCHROME-P-450; SALICYLATE; GLYCINE; MITOCHONDRIAL; REOXYGENATION; PRODUCTS; TUBULES; FLOW;
Keywords:
hippurate; benzoate; hydroxyl radical; hydroxybenzoates;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Malyusz, M Univ Kiel, Inst Physiol, Hermann Rodewald Str 5, D-24118 Kiel, Germany Univ Kiel Hermann Rodewald Str 5 Kiel Germany D-24118 Germany
Citazione:
M. Malyusz et al., "Hippurate metabolism as a hydroxyl radical trapping mechanism in the rat kidney", KIDNEY BL P, 24(3), 2001, pp. 149-158

Abstract

Hippurate (Hip) is considered to be the end product of benzoate (BA) metabolism. However, the kidney is able to metabolize Hip. Although only Hip butno BA is present in the blood, rat urine contains under normal conditions less Hip (about 0.4 mM) than BA (about 4.5 mM) and of hydroxylated derivatives of BA (hydroxyBAs = HB and dihydroxy-BAs = DHIB). Generation of HBs andDHBs is the result of radical substitution by free OH radicals COH). Thus,rate of synthesis of HBs and DH13s may reflect the production rate of (OH)-O-. in the kidney. (OH)-O-. generation is elevated following ischemic stress. Therefore, production of HBs and DHBs can be expected to be elevated inpostischemic injury. The validity of this assumption was tested in vitro on isolated tubular segments and in vivo in the rat. Metabolism of Hip at 0.1 mmol l(-1) (0.1 mM) as well as of BA resulted in enlarged production of both HBs (especially 3-HB and 4-HB) and of DHBs (especially 2,6-DHB). Production of 2,3-and especially of 2,5-DHB was elevated in the presence of high concentration (1.0 mM) of salicylate (2-HB) only. In vivo both in acute (120 min) and in chronic (5 days) experiments ligation of one renal artery for30 respectively 60 min resulted in enlarged excretion of HBs and DHBs, especially of 2,6- and 3,5-DHB. This finding is noteworthy since (a) formationof 2,6-DHB necessitates as precursor salicylate which could not be detected in our experiments and (b) the spontaneous attack of (OH)-O-. upon the benzol ring would prefer the positions 2,3- 2,5-and 3,4-. Therefore, the existence of regulating factor(s) guiding OH groups to definite positions is a distinct possibility. These results indicate that metabolism of Hip leadingto hydroxylated BAs may be a renoprotective mechanism against attack of (OH)-O-. in reoxygenated renal tissue. Copyright (C) 2001 S. Karger AG. Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 02:51:58