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Titolo:
Engraftment of NOD/SCID mice with human CD34(+) cells transduced by concentrated oncoretroviral vector particles pseudotyped with the feline endogenous retrovirus (RD 114) envelope protein
Autore:
Gatlin, J; Melkus, MW; Padgett, A; Kelly, PF; Garcia, JV;
Indirizzi:
Univ Texas, SW Med Ctr, Dept Internal Med, Div Infect Dis Y9206, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 Div Infect Dis Y9206, Dallas, TX 75390 USA St Jude Childrens Hosp, Dept Hematol, Memphis, TN 38105 USA St Jude Childrens Hosp Memphis TN USA 38105 ematol, Memphis, TN 38105 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 20, volume: 75, anno: 2001,
pagine: 9995 - 9999
SICI:
0022-538X(200110)75:20<9995:EONMWH>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; GENE-TRANSFER VECTORS; LONG-TERM CULTURES; REPOPULATING CELLS; EFFICIENT TRANSDUCTION; LENTIVIRAL VECTORS; HIGHLY EFFICIENT; IMMUNODEFICIENT MICE; IN-VITRO; VIVO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Garcia, JV Univ Texas, SW Med Ctr, Dept Internal Med, Div Infect Dis Y9206, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 Dis Y9206, Dallas, TX 75390 USA
Citazione:
J. Gatlin et al., "Engraftment of NOD/SCID mice with human CD34(+) cells transduced by concentrated oncoretroviral vector particles pseudotyped with the feline endogenous retrovirus (RD 114) envelope protein", J VIROLOGY, 75(20), 2001, pp. 9995-9999

Abstract

Oncoretrovirus vectors pseudotyped with the feline endogenous retrovirus (RD114) envelope protein produced by the FLYRD18 packaging cell line have previously been shown to transduce human hematopoietic progenitor cells with a greater efficiency than similar amphotropic envelope-pseudotyped vectors. In this report, we describe the production and efficient concentration of RD114-pseudotyped murine leukemia virus (MLV)-based vectors. Following a single round of centrifugation, vector supernatants were concentrated approximately 200-fold with a 50 to 70% yield. Concentrated vector stocks transduced prestimulated human CD34(+) (hCD34(+)) cells with approximately 69% efficiency (n = 7, standard deviation = 4.4%) using a single addition of vectorat a low multiplicity of infection (MOI = 5). Introduction of transduced hCD34(+) cells into irradiated NOD/SCID recipients resulted in multilineage engraftment with long-term transgene expression. These data demonstrate that RD114-pseudotyped MLV-based vectors can be efficiently concentrated to high titers and that hCD34(+) cells transduced with concentrated vector stocks retain in vivo repopulating potential. These results highlight the potential of RD114-pseudotyped oncoretrovirus vectors for future clinical implementation in hematopoietic stem cell gene transfer.

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Documento generato il 24/11/20 alle ore 14:35:34