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Titolo:
The use of a neutralizing monoclonal antibody to detect infections of equine herpesvirus type 2 (EHV-2)
Autore:
Nordengrahn, A; Klingeborn, B; Lindholm, A; Merza, M;
Indirizzi:
Natl Vet Inst, SVANOVA Biotech, S-75183 Uppsala, Sweden Natl Vet Inst Uppsala Sweden S-75183 VA Biotech, S-75183 Uppsala, Sweden Natl Vet Inst, Dept Virol, S-75123 Uppsala, Sweden Natl Vet Inst UppsalaSweden S-75123 Dept Virol, S-75123 Uppsala, Sweden Univ Agr Sci Uppsala, Dept Med & Surg, Uppsala, Sweden Univ Agr Sci Uppsala Uppsala Sweden a, Dept Med & Surg, Uppsala, Sweden
Titolo Testata:
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
fascicolo: 5, volume: 13, anno: 2001,
pagine: 389 - 393
SICI:
1040-6387(200109)13:5<389:TUOANM>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCOPROTEIN-B; FOALS; VIRUS; EPIZOOTIOLOGY; DISEASE; HORSES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Nordengrahn, A Natl Vet Inst, SVANOVA Biotech, Uppsala Sci Pk,Glunten, S-75183 Uppsala, Sweden Natl Vet Inst Uppsala Sci Pk,Glunten Uppsala Sweden S-75183
Citazione:
A. Nordengrahn et al., "The use of a neutralizing monoclonal antibody to detect infections of equine herpesvirus type 2 (EHV-2)", J VET D INV, 13(5), 2001, pp. 389-393

Abstract

A blocking enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to equine herpesvirus 2 in serum samples of horses. By measuring the binding to a single epitope, this blocking ELISA gives a good picture of the antibody status in the animal. The test is based on a monoclonal antibody with neutralizing activity and had a sensitivity of 94% and a specificity of 100%. Antibodies due to newly acquired infection in foals were successfully detected with this blocking ELISA.

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Documento generato il 25/01/20 alle ore 03:18:25