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Titolo:
An in vitro assay to detect Paralytic Shellfish poison
Autore:
Cordova, JL; Jamett, A; Aguayo, J; Faure, MT; Villarroel, O; Cardenas, L;
Indirizzi:
Fdn Ciencia Vida, Santiago, Chile Fdn Ciencia Vida Santiago ChileFdn Ciencia Vida, Santiago, Chile Millennium Inst Fundamental & Appl Biol, Santiago, Chile Millennium Inst Fundamental & Appl Biol Santiago Chile Santiago, Chile BiosChile IGSA, Santiago, Chile BiosChile IGSA Santiago ChileBiosChile IGSA, Santiago, Chile Serv Salud Magallanes, Punta Arenas, Chile Serv Salud Magallanes Punta Arenas Chile agallanes, Punta Arenas, Chile Inst Salud Publ Chile, Lab Ambiente, Santiago, Chile Inst Salud Publ Chile Santiago Chile ile, Lab Ambiente, Santiago, Chile
Titolo Testata:
JOURNAL OF SHELLFISH RESEARCH
fascicolo: 1, volume: 20, anno: 2001,
pagine: 55 - 61
SICI:
0730-8000(200106)20:1<55:AIVATD>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE BIOASSAY; SAXITOXIN; TOXINS; RADIOIMMUNOASSAY; TETRODOTOXIN;
Keywords:
Paralytic shellfish poison; saxitoxin; monoclonal antibodies; dinoflagellates; assay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Cordova, JL Fdn Ciencia Vida, Av Marathon 1943, Santiago, Chile Fdn Ciencia Vida Av Marathon 1943 Santiago Chile iago, Chile
Citazione:
J.L. Cordova et al., "An in vitro assay to detect Paralytic Shellfish poison", J SHELLFISH, 20(1), 2001, pp. 55-61

Abstract

We have developed an in vitro assay named Paralytic Shellfish Poison-test (PSP-test) that is capable of detecting low quantities of saxitoxin (STX), the first toxin studied from PSP. The PSP-test is an immunoassay based on the development of specific monoclonal antibodies (MAbs) capable of recognizing soluble saxitoxin (STX) and able to agglutinate in minutes, a latex containing STX adsorbed to its surface. The PSP-test is a two-step indirect immunoassay. During the first step, the MAbs are incubated with an extract suspected of containing STX. If the solution contains STX, this molecule willoccupy the MAbs binding sites and block the latex agglutination. In the second step, MAbs-STX are incubated with an STX-latex, An inhibition indicates the presence of STX (PSP) in the sample. The PSP-test can detect within the range of 1.25-2.5 mug/ml of STX standard. It is also capable of recognizing STX in whole shellfish extract, acidic shellfish extract, and supernatants from aged toxic culture,.; of bacteria or dinoflagellates. The assay issimple, economic, and quick. Training of in its use is fast and harmless to the user. Tt can be performed in the field as a first screening procedurebecause an instrument to interpret the results is not required. However, the PSP-test was not developed to replace the mouse bioassay but rather to be complementary, and the strategy used for its development can be easily adapted to other toxins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/08/20 alle ore 14:50:32