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Titolo:
mRNA markers of breast cancer nodal metastases: comparison between mammaglobin and carcinoembryonic antigen in 248 patients
Autore:
Marchetti, A; Buttitta, F; Bertacca, G; Zavaglia, K; Bevilacqua, G; Angelucci, D; Viacava, P; Naccarato, A; Bonadio, A; Barassi, F; Felicioni, L; Salvatore, S; Mucilli, F;
Indirizzi:
Univ Gabriele DAnnunzio, Dept Oncol & Neurosci, I-66100 Chieti, Italy UnivGabriele DAnnunzio Chieti Italy I-66100 osci, I-66100 Chieti, Italy Univ Pisa, Dept Oncol, I-56100 Pisa, Italy Univ Pisa Pisa Italy I-56100Univ Pisa, Dept Oncol, I-56100 Pisa, Italy Univ Gabriele DAnnunzio, Dept Surg, I-66100 Chieti, Italy Univ Gabriele DAnnunzio Chieti Italy I-66100 Surg, I-66100 Chieti, Italy
Titolo Testata:
JOURNAL OF PATHOLOGY
fascicolo: 2, volume: 195, anno: 2001,
pagine: 186 - 190
SICI:
0022-3417(200109)195:2<186:MMOBCN>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-CHAIN-REACTION; AXILLARY LYMPH-NODES; MICROMETASTASES; GENE;
Keywords:
breast; carcinoma; mammaglobin; carcinoembryonic antigen; RT-PCR; metastasis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Marchetti, A Osped Clinicizzato, Mol Pathol Sect, Dept Oncol & Neurosci, Via Vestini, I-66013 Chieti, Italy Osped Clinicizzato Via Vestini Chieti Italy I-66013 i, Italy
Citazione:
A. Marchetti et al., "mRNA markers of breast cancer nodal metastases: comparison between mammaglobin and carcinoembryonic antigen in 248 patients", J PATHOLOGY, 195(2), 2001, pp. 186-190

Abstract

Histological detection of axillary lymph node metastases is still the mostvaluable prognostic parameter for breast cancer, but about 30%, of node-negative patients relapse within five years, suggesting that current methods are inadequate for identifying metastatic disease. More sensitive, PCR-based methods for the detection of metastatic cells are now available, enablingthe amplification of cancer cell-specific mRNA messages by the RT-PCR assay. An ideal tumour marker, consistently expressed in tumour samples and notat all in normal lymph nodes, remains to be identified. The present study first investigated the expression of seven mRNA markers, CEA, CK19, c-Met, mammaglobin, MUC-1, beta1 --> GalNAc-T and p97, selected on the basis of their previously reported specificity for breast cancer cells. Eighteen lymphnodes were examined from patients without tumours. Only mammaglobin mRNA and CEA mRNA were not expressed in normal nodes. All of the other markers showed a band of expression in ITV 55% of cases, indicating that they are notbreast cancer-specific. CEA mRNA and mammaglobin mRNA expression could be detected in 15/20 (75%) and 19/20 (95%,,) primary breast carcinomas, respectively. The expression of mammaglobin mRNA and CEA mRNA was then compared in axillary lymph nodes from 248 consecutive breast cancer patients, 89 withhistologically documented lymph node metastasis and 159 without histological evidence of metastatic disease. Ninety-seven per cent of the patients with histologically involved nodes showed expression of mammaglobin mRNA, whereas CEA mRNA was expressed in 79%, of these cases. In the group of patients with histologically negative lymph nodes, 46 (29%) and 32 (20%) were found to be positive for mammaglobin and CEA expression, respectively, indicating the presence of metastases not detected by routine histological examination of one lymph node section. These results show that both mammaglobin RT-PCR and CEA RT-PCR are useful tools for the detection of breast cancer metastases in axillary lymph nodes. The detection sensitivity of the mammaglobin RT-PCR is far superior to that of the CEA RT-PCR, allowing the diagnosis of occult metastases in nearly one-third of cases. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 11:31:03