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Titolo:
Simultaneous measurement of cerebral blood flow and mRNA signals: pixel-based inter-modality correlational analysis
Autore:
Zhao, WZ; Busto, R; Truettner, J; Ginsberg, MD;
Indirizzi:
Univ Miami, Sch Med, Cerebral Vasc Dis Res Ctr, Dept Neurol D4 5, Miami, FL 33101 USA Univ Miami Miami FL USA 33101 Ctr, Dept Neurol D4 5, Miami, FL 33101 USA Univ Miami, Coll Engn, Cerebral Vasc Dis Res Ctr, Dept Biomed Engn, Miami,FL USA Univ Miami Miami FL USA Vasc Dis Res Ctr, Dept Biomed Engn, Miami,FL USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE METHODS
fascicolo: 2, volume: 108, anno: 2001,
pagine: 161 - 170
SICI:
0165-0270(20010730)108:2<161:SMOCBF>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-RESOLUTION 2-DEOXYGLUCOSE; DOUBLE-LABEL AUTORADIOGRAPHY; HSP70 MESSENGER-RNA; GLUCOSE-UTILIZATION; GENE-EXPRESSION; ARTERY OCCLUSION; C-FOS; ISCHEMIA; RAT; DEOXYGLUCOSE;
Keywords:
mRNA expression; in situ hybridization; cerebral blood flow; autoradiography; image-processing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Zhao, WZ Univ Miami, Sch Med, Cerebral Vasc Dis Res Ctr, Dept Neurol D4 5,1501 NW 9th Ave,POB 016960, Miami, FL 33101 USA Univ Miami 1501 NW 9th Ave,POB 016960 Miami FL USA 33101 3101 USA
Citazione:
W.Z. Zhao et al., "Simultaneous measurement of cerebral blood flow and mRNA signals: pixel-based inter-modality correlational analysis", J NEUROSC M, 108(2), 2001, pp. 161-170

Abstract

The analysis of pixel-based relationships between local cerebral blood flow (LCBF) and mRNA expression can reveal important insights into brain function. Traditionally, LCBF and in situ hybridization studies for genes of interest have been analyzed in separate series. To overcome this limitation and to increase the power of statistical analysis, this study focused on developing a double-label method to measure local cerebral blood flow (LCBF) and gene expressions simultaneously by means of a dual-autoradiography procedure. A C-14-iodoantipyrine autoradiographic LCBF study was first performed. Serial brain sections (12 in this study) were obtained at multiple coronallevels and were processed in the conventional manner to yield quantitativeLCBF images. Two replicate sections at each bregma level were then used for in situ hybridization. To eliminate the C-14-iodoantipyrine from these sections, a chloroform-washout procedure was first performed. The sections were then processed for in situ hybridization autoradiography for the probes of interest. This method was tested in Wistar rats subjected to 12 min of global forebrain ischemia by two-vessel occlusion plus hypotension, followedby 2 or 6 h of reperfusion (n = 4-6 per group). LCBF and in situ hybridization images for heat shock protein 70 (HSP70) were generated for each rat, aligned by disparity analysis, and analyzed on a pixel-by-pixel basis. Thismethod yielded detailed inter-modality correlation between LCBF and HSP70 mRNA expressions. The advantages of this method include reducing the numberof experimental animals by one-half; and providing accurate pixel-based correlations between different modalities in the same animals, thus enabling paired statistical analyses. This method can be extended to permit correlation of LCBF with the expression of multiple genes of interest. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 31/03/20 alle ore 18:37:32