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Titolo:
Statistical aspects of quantitative image analysis of beta-amyloid in the APP(V717F) transgenic mouse model of Alzheimer's disease
Autore:
Fishman, CE; Cummins, DJ; Bales, KR; DeLong, CA; Esterman, MA; Hanson, JC; White, SL; Paul, SM; Jordan, WH;
Indirizzi:
Lilly Res Labs, Div Toxicol Pathol, Greenfield, IN 46140 USA Lilly Res Labs Greenfield IN USA 46140 l Pathol, Greenfield, IN 46140 USA Lilly Res Labs, Div Stat & Math Sci, Indianapolis, IN 46285 USA Lilly Res Labs Indianapolis IN USA 46285 Sci, Indianapolis, IN 46285 USA Purdue Univ, Sch Vet Med, Dept Vet Pathobiol, W Lafayette, IN 47907 USA Purdue Univ W Lafayette IN USA 47907 Pathobiol, W Lafayette, IN 47907 USA Lilly Res Labs, Div Neurosci, Indianapolis, IN 46285 USA Lilly Res Labs Indianapolis IN USA 46285 osci, Indianapolis, IN 46285 USA Lilly Res Labs, Div Informat Technol Discovery, Indianapolis, IN 46285 USALilly Res Labs Indianapolis IN USA 46285 very, Indianapolis, IN 46285 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE METHODS
fascicolo: 2, volume: 108, anno: 2001,
pagine: 145 - 152
SICI:
0165-0270(20010730)108:2<145:SAOQIA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRECURSOR PROTEIN; APOLIPOPROTEIN-E; PEPTIDE DEPOSITION; NEURONAL LOSS; MICE; PATHOLOGY; PLAQUES; BRAIN; PRESENILIN-1;
Keywords:
Alzheimer's disease; animal model; beta-amyloid; histopathology; image analysis; mouse; power analysis; quantitative;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Jordan, WH Lilly Res Labs, Div Toxicol Pathol, POB 708, Greenfield, IN 46140 USA Lilly Res Labs POB 708 Greenfield IN USA 46140 ld, IN 46140 USA
Citazione:
C.E. Fishman et al., "Statistical aspects of quantitative image analysis of beta-amyloid in the APP(V717F) transgenic mouse model of Alzheimer's disease", J NEUROSC M, 108(2), 2001, pp. 145-152

Abstract

Cerebral beta -amyloidosis is a central part of the neuropathology of Alzheimer's disease (AD). Quantitation of beta -amyloid plaques in the human ADbrain, and in animal models of AD, is an important study endpoint in AD research. Methodologic approaches to the measurement of beta -amyloid in the brain vary between investigators, and these differences affect outcome measures. Here, one quantitative approach to the measurement of beta -amyloid plaques in brain sections was analyzed for sources of variability due to sampling. Brain tissue was from homozygous APP(V717F) transgenic male mice. Sampling variables were at the mouse and microscopic slide and field levels. Results indicated that phenotypic variability in the mouse sample population was the largest contributor to the standard error of the analyses. Withineach mouse, variability between slides or between fields within slides hadsmaller effects on the error of the analyses. Therefore, when designing studies of adequate power, in this and in other similar models of cerebral beta -amyloidosis, sufficient numbers of mice per group must be included in order for change in mean plaque burden attributable to an experimental variable to outweigh phenotypic variability. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 00:30:30