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Titolo:
Swelling of capillary endothelial cells and cardiomyocytes in the ischaemic myocardium of artificially arrested canine hearts
Autore:
Schmiedl, A; Schnabel, PA; von Schmeling, HKB; Marten, K; Richter, J;
Indirizzi:
Univ Hannover, Sch Med, Dept Anat, D-30625 Hannover, Germany Univ Hannover Hannover Germany D-30625 t Anat, D-30625 Hannover, Germany Dept Anat, Div Electron Microscopy, D-37075 Gottingen, Germany Dept Anat Gottingen Germany D-37075 croscopy, D-37075 Gottingen, Germany Univ Heidelberg, Dept Pathol, D-69120 Heidelberg, Germany Univ HeidelbergHeidelberg Germany D-69120 , D-69120 Heidelberg, Germany Cent Hosp Bremen Nord, Dept Surg, D-28755 Bremen, Germany Cent Hosp BremenNord Bremen Germany D-28755 rg, D-28755 Bremen, Germany
Titolo Testata:
JOURNAL OF ELECTRON MICROSCOPY
fascicolo: 4, volume: 50, anno: 2001,
pagine: 349 - 357
SICI:
0022-0744(2001)50:4<349:SOCECA>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISCHEMIC CARDIAC-ARREST; NO-REFLOW PHENOMENON; CARDIOPLEGIC SOLUTIONS; PROTECTION; PRESERVATION; RAT; HYPOTHERMIA; OCCLUSION; PERFUSION; STORAGE;
Keywords:
ultrastructure; cardioplegia; hypothermia; ischaemia; myocardial protection; stereology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Schmiedl, A Univ Hannover, Sch Med, Dept Anat, Carl Neuberg Str 1, D-30625Hannover, Germany Univ Hannover Carl Neuberg Str 1 Hannover Germany D-30625 any
Citazione:
A. Schmiedl et al., "Swelling of capillary endothelial cells and cardiomyocytes in the ischaemic myocardium of artificially arrested canine hearts", J ELEC MICR, 50(4), 2001, pp. 349-357

Abstract

To establish whether coronary perfusion with cardioplegic solutions results in better intraischaemic structural preservation of endothelial cells than of cardiomyocytes, we determined intraischaemic swelling of these two cell types in hearts differently arrested during global ischaemia at 5 degreesC. Cardiac arrest was induced in situ by aortic cross clamping or by additional coronary perfusion with various cardioplegic solutions. Parameters forcellular swelling were determined, i.e. barrier thickness of capillary endothelial cells and sum of the volume fractions (V-V) of free sarcoplasm andmitochondria (V-VSp + V-VMi) in cardiomyocytes. in order to test the intraischaemic relative increase of cellular volume in both cell types, regression analyses were performed. The results show that the relative intraischaemic volume increase was similar in both cell types after perfusion with histidine-tryptophan-ketoglutarate solution, and significantly less pronounced in capillary endothelial cells after perfusion with University of Wisconsinsolution. in hearts arrested with St. Thomas' Hospital solution, a significantly higher volume increase was determined in capillary endothelial cells. Thus, capillary endothelium does not generally show a higher structural preservation than cardiomyocytes during ischaemia. instead, volume regulation in both types of cells depends on the type of cardioplegic solution used. These results should be taken into consideration in human transplantation medicine.

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Documento generato il 04/12/20 alle ore 09:49:50