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Titolo:
Pentosidine in advanced glycation end-products (AGEs) during UVA irradiation generates active oxygen species and impairs human dermal fibroblasts
Autore:
Okano, Y; Masaki, H; Sakurai, H;
Indirizzi:
Noevir Co Ltd, Kobe Res Labs, Chuo Ku, Kobe, Hyogo 6508521, Japan Noevir Co Ltd Kobe Hyogo Japan 6508521 huo Ku, Kobe, Hyogo 6508521, Japan Kyoto Pharmaceut Univ, Dept Analyt & Bioinorgan Chem, Yamashina Ku, Kyoto 607, Japan Kyoto Pharmaceut Univ Kyoto Japan 607 em, Yamashina Ku, Kyoto 607, Japan
Titolo Testata:
JOURNAL OF DERMATOLOGICAL SCIENCE
, volume: 27, anno: 2001, supplemento:, 1
pagine: S11 - S18
SICI:
0923-1811(200108)27:<S11:PIAGE(>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-SKIN COLLAGEN; MAILLARD REACTION; IN-VITRO; DIABETES-MELLITUS; SUPEROXIDE ANION; PROTEINS; RADICALS; IDENTIFICATION; COMPLICATIONS; DISEASE;
Keywords:
pentosidine; advanced glycation end-products; fibroblasts; UVA; ESR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Masaki, H Noevir Co Ltd, Kobe Res Labs, Chuo Ku, 13-1 Port Isl,Naka Machi 6 Chome, Kobe, Hyogo 6508521, Japan Noevir Co Ltd 13-1 Port Isl,Naka Machi 6 Chome Kobe Hyogo Japan 6508521
Citazione:
Y. Okano et al., "Pentosidine in advanced glycation end-products (AGEs) during UVA irradiation generates active oxygen species and impairs human dermal fibroblasts", J DERMA SCI, 27, 2001, pp. S11-S18

Abstract

Our previous study reported that advanced glycation end-products (AGE)-modified BSA produced active oxygen species, O-.(2)-. H2O2, and (OH)-O-. underUVA irradiation and enhanced the cytotoxicity of UVA light. We examined whether pentosidine in AGE-modified BSA was involved in one of the mechanismsgenerating the active oxygen species. In biological investigations, fibroblasts exposed to UVA (20 J/cm(2)) in the presence of pentosidine-rich compounds (PRCs), which were prepared with L-arginine, L-lysine and glucose, showed a time-dependent leakage of the cytosolic enzyme LDH. In addition, release of LDH was suppressed by addition of DMSO and deferoxamine under UVA irradiation. From these results, it was determined that PRCs exposed to UVA damaged the plasma membrane of human dermal fibroblasts due to the conversion of (OH)-O-. from H2O2 via a Fenton-like reaction. These features of PRCs exposed to UVA were consistent with those of AGE-modified BSA. In an ESR study, PRCs under UVA irradiation yielded DMPO-OH (DMPO-OH adduct) using DMPOas a spin-trapping reagent. O-.(2) generation from UVA-irradiated PRCs wasalso indicated by the combination of NBT reduction and SOD. When PRCs wereexposed to UVA light controlled with a long-pass filter, WG-360, it was found that their production of O-.(2)- was prohibited less than 50% in the NBT reduction assay. The O-.(2)- production profile of PRCs depending on the wavelength of UVA light was similar to that of AGE-modified BSA. Furthermore, it was found that the H2O2 level was increased by PRCs exposed to UVA. These results indicated that pentosidine is an important factor of AGE-modified BSA in active oxygen generation under UVA irradiation. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 15:08:37