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Titolo:
Quantitative PCR determination of human cytomegalovirus in blood cells
Autore:
Satou, J; Funato, S; Satoh, N; Abe, Y; Ishii, KK; Sasaki, T; Kaku, M;
Indirizzi:
Tohoku Univ, Sch Med, Dept Clin Med, Div Mol Diagnost,Aoba Ku, Sendai, Miyagi 9808574, Japan Tohoku Univ Sendai Miyagi Japan 9808574 Ku, Sendai, Miyagi 9808574, Japan Tohoku Univ, Sch Med, Dept Clin Med, Div Rheumatol & Haematol, Sendai, Miyagi 9808574, Japan Tohoku Univ Sendai Miyagi Japan 9808574 ol, Sendai, Miyagi 9808574, Japan
Titolo Testata:
JOURNAL OF CLINICAL LABORATORY ANALYSIS
fascicolo: 3, volume: 15, anno: 2001,
pagine: 122 - 126
SICI:
0887-8013(2001)15:3<122:QPDOHC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; MESSENGER-RNA; DNA; PRODUCT; VIRUS;
Keywords:
polymerase chain reaction (PCR); quantitative PCR; cytomegalovirus (CMV); liver transplantation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Funato, S Tohoku Univ, Sch Med, Dept Clin Med, Div Mol Diagnost,Aoba Ku, 1-1 Seiryo Machi, Sendai, Miyagi 9808574, Japan Tohoku Univ 1-1 Seiryo MachiSendai Miyagi Japan 9808574 , Japan
Citazione:
J. Satou et al., "Quantitative PCR determination of human cytomegalovirus in blood cells", J CL LAB AN, 15(3), 2001, pp. 122-126

Abstract

We evaluated a rapid and sensitive method to determine human cytomegalovirus (CMV) DNA levels in blood cells using a quantitative polymerase chain reaction (PCR) technique. This method is based on real-time detection of PCR using a dual fluorescence-labeled probe and a sequence detector. Ten copiesof CMV DNA were detected, when 1 mug of DNA from blood samples was used with this method, and a good correlation was obtained between increased concentrations of copy numbers calculated and measured copy numbers of CMV DNA (r = 0.999). Forty normal subjects exhibited no copies of CMV DNA. On the other hand, a 6-month-old girl tested positive for increased levels 4 weeks after liver transplant. This method is simple, accurate, and sensitive for the quantitative detection of CMV DNA in vivo, indicating possible applications for the diagnosis and monitoring of CMV infection. J. Clin. Lab. Anal. 15:122-126, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:35:46