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Titolo:
High-performance liquid chromatographic assay for the determination of 2 '-deoxy-3 '-thiacytidine (lamivudine) in human plasma
Autore:
Zheng, JJ; Wu, ST; Emm, TA;
Indirizzi:
Dupont Merck Pharmaceut Co, Drug Metab & Pharmacokinet Sect, Newark, DE 19714 USA Dupont Merck Pharmaceut Co Newark DE USA 19714 Sect, Newark, DE 19714 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 761, anno: 2001,
pagine: 195 - 201
SICI:
1387-2273(20010925)761:2<195:HLCAFT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; TANDEM MASS-SPECTROMETRY; HUMAN SERUM; ZIDOVUDINE; (-)-2'-DEOXY-3'-THIACYTIDINE; THERAPY;
Keywords:
2 '-deoxy-3 '-thiacytidine; lamivudine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Zheng, JJ Dupont Merck Pharmaceut Co, Drug Metab & Pharmacokinet Sect, Elkton Rd,POB30,S115, Newark, DE 19714 USA Dupont Merck Pharmaceut Co Elkton Rd,POB 30,S115 Newark DE USA 19714
Citazione:
J.J. Zheng et al., "High-performance liquid chromatographic assay for the determination of 2 '-deoxy-3 '-thiacytidine (lamivudine) in human plasma", J CHROMAT B, 761(2), 2001, pp. 195-201

Abstract

A method for the quantification of 2 ' -deoxy-3 ' -thiacytidine (lamivudine, 3-TC), which incorporated the use of 3-isobutyl-methylxanthine as internal standard (I.S.) was developed and validated in human plasma, using HPLC with UV absorbance detection. Using solid-phase extraction, 3-TC and I.S. were selectively extracted from human plasma. Subsequently, chromatographic separation was performed using a YMC phenyl column with ion-pair chromatography and detection at 270 nm. The method was validated over a concentrationrange of 10 to 5000 ng/ml using 0.5 ml of human plasma. The extraction recovery for both 3-TC and I.S. was greater than 95%. The determination of inter- and intra-day precision (RSD) was less than 10% at all concentration levels, while the inter- and intra-day accuracy (% difference) was less than 6%. (C) 2001 DuPont Pharmaceuticals Company. Published by Elsevier Science B.V. All rights reserved.

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Documento generato il 01/04/20 alle ore 09:07:16