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Titolo:
High-performance liquid chromatographic method to screen and quantitate seven selective serotonin reuptake inhibitors in human serum
Autore:
Tournel, G; Houdret, N; Hedouin, V; Deveaux, M; Gosset, D; Lhermitte, M;
Indirizzi:
Univ Lille 2, Inst Med Legale Lille, Fac Med, F-59000 Lille, France Univ Lille 2 Lille France F-59000 Lille, Fac Med, F-59000 Lille, France Hop Calmette, Toxicol Lab, F-59000 Lille, France Hop Calmette Lille France F-59000 te, Toxicol Lab, F-59000 Lille, France
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 761, anno: 2001,
pagine: 147 - 158
SICI:
1387-2273(20010925)761:2<147:HLCMTS>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE EXTRACTION; HUMAN-PLASMA; ULTRAVIOLET DETECTION; FLUORESCENCE DETECTION; COULOMETRIC DETECTION; CITALOPRAM; PAROXETINE; METABOLITES; NORFLUOXETINE; FLUOXETINE;
Keywords:
fluvoxamine; milnacipran; paroxetine; sertraline; fluoxetine; citalopram; venlafaxine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Tournel, G Univ Lille 2, Inst Med Legale Lille, Fac Med, Pl Theo Varlet, F-59000 Lille, France Univ Lille 2 Pl Theo Varlet Lille France F-59000 Lille, France
Citazione:
G. Tournel et al., "High-performance liquid chromatographic method to screen and quantitate seven selective serotonin reuptake inhibitors in human serum", J CHROMAT B, 761(2), 2001, pp. 147-158

Abstract

A high-performance liquid chromatographic screening method (HPLC) is described for the determination of seven selective serotonin reuptake inhibitors(SSRIs) (fluvoxamine, milnacipran, paroxetine, sertraline, fluoxetine, citalopram, venlafaxine) and for three pharmacologically active N-demethylatedmetabolites (desmethylcitalopram, didesmethylcitalopram and norfluoxetine). A tricyclic antidepressant, clomipramine, was used as an internal standard. The method consists of liquid extraction of serum after alcalinisation at pH 9.50, followed by chromatography on a Beckman C-18 reversed-phase column. Compounds were detected at 200.4 nm. The standard curves were linear over a working range of 50-1000 ng/ml for fluvoxamine, 15-1000 ng/ml for fluoxetine, 25-500 ng/nd for norfluoxetine, 50-500 ng/ml for sertraline, 20-500ng/ml for paroxetine, 25-550 ng/ml for citalopram, 25-750 ng/ml for desmethylcitalopram, 25-800 ng/ml for didesmethylcitalopram, 25-650 ng/ml for milnacipran, and 25-500 ng/ml for venlafaxine. The quantitation limits of the method were 15 ng/ml for fluoxetine, 20 ng/ml for paroxetine, 25 ng/ml for venlafaxine, norfluoxetine and citalopram, and its metabolites, 40 ng/ml for sertraline and 50 ng/ml for fluvoxamine. No interferences were noted withthis sensitive and specific method which can be used for therapeutic drug monitoring. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 19:27:12