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Titolo:
Accelerated up-regulation of L-Sox5, Sox6, and Sox9 by BMP-2 gene transferduring murine fracture healing
Autore:
Uusitalo, H; Hiltunen, A; Ahonen, M; Gao, TJ; Lefebvre, V; Harley, V; Kahari, VM; Vuorio, E;
Indirizzi:
Univ Turku, Dept Med Biochem & Mol Biol, Skeletal Res Program, FIN-20520 Turku, Finland Univ Turku Turku Finland FIN-20520 Res Program, FIN-20520 Turku, Finland Univ Turku, Cent Hosp, Dept Surg, FIN-20520 Turku, Finland Univ Turku Turku Finland FIN-20520 , Dept Surg, FIN-20520 Turku, Finland Univ Turku, Cent Hosp, Dept Dermatol, FIN-20520 Turku, Finland Univ TurkuTurku Finland FIN-20520 pt Dermatol, FIN-20520 Turku, Finland Univ Turku, Turku Ctr Biotechnol, Turku, Finland Univ Turku Turku Finland iv Turku, Turku Ctr Biotechnol, Turku, Finland Abo Akad Univ, Turku, Finland Abo Akad Univ Turku FinlandAbo Akad Univ, Turku, Finland Univ Texas, MD Anderson Canc Ctr, Dept Mol Genet, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 tr, Dept Mol Genet, Houston, TX 77030 USA Univ Melbourne, Howard Florey Inst Expt Physiol & Med, Parkville, Vic 3052, Australia Univ Melbourne Parkville Vic Australia 3052 arkville, Vic 3052, Australia
Titolo Testata:
JOURNAL OF BONE AND MINERAL RESEARCH
fascicolo: 10, volume: 16, anno: 2001,
pagine: 1837 - 1845
SICI:
0884-0431(200110)16:10<1837:AUOLSA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
AUTOSOMAL SEX REVERSAL; CHONDROCYTE-SPECIFIC ENHANCER; COLLAGEN MESSENGER-RNAS; BONE MORPHOGENETIC PROTEIN; SRY-RELATED GENE; CAMPOMELIC DYSPLASIA; II COLLAGEN; MONOCLONAL-ANTIBODIES; HYBRIDIZATION PROBES; RETINOIC ACID;
Keywords:
chondrocyte; fracture; transcription factor Sox; bone morphogenetic protein 2; mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Vuorio, E Univ Turku, Dept Med Biochem & Mol Biol, Skeletal Res Program, Kiinamyllynkatu 10, FIN-20520 Turku, Finland Univ Turku Kiinamyllynkatu 10 Turku Finland FIN-20520 , Finland
Citazione:
H. Uusitalo et al., "Accelerated up-regulation of L-Sox5, Sox6, and Sox9 by BMP-2 gene transferduring murine fracture healing", J BONE MIN, 16(10), 2001, pp. 1837-1845

Abstract

Fracture repair is the best-characterized situation in which activation ofchondrogenesis takes place in an adult organism. To better understand the mechanisms that regulate chondrogenic differentiation of mesenchymal progenitor cells during fracture repair, we have investigated the participation of transcription factors L-Sox5, Sox6, and Sox9 in this process. Marked up-regulation of L-Sox5 and Sox9 messenger RNA (mRNA) and smaller changes in Sox6 mRNA levels were observed in RNAse protection assays during early stagesof callus formation, followed by up-regulation of type II collagen production. During cartilage expansion, the colocalization of L-Sox5, Sox6, and Sox9 by immunohistochemistry and type II collagen transcripts by in situ hybridization confirmed a close relationship of these transcription factors with the chondrocyte phenotype and cartilage production. On chondrocyte hypertrophy, production of L-Sox5, Sox9 and type II collagen were down-regulated markedly and that of type X collagen was up-regulated. Finally, using adenovirus mediated bone morphogenetic protein 2 (BMP-2) gene transfer into fracture site we showed accelerated up-regulation of the genes for all three Sox proteins and type II collagen in fractures treated with BMP-2 when compared with control fractures. These data suggest that L-Sox5, Sox6, and Sox9 are involved in the activation and maintenance of chondrogenesis during fracture healing and that enhancement of chondrogenesis by BMP-2 is mediated via an L-Sox5/Sox6/Sox9-dependent pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 13:11:59