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Titolo:
Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6
Autore:
Dilcher, M; Kohler, B; von Mollard, GF;
Indirizzi:
Univ Gottingen, Biochem Abt 2, Zentrum Biochem & Mol Zellbiol, D-37073 Gottingen, Germany Univ Gottingen Gottingen Germany D-37073 iol, D-37073 Gottingen, Germany
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 37, volume: 276, anno: 2001,
pagine: 34537 - 34544
SICI:
0021-9258(20010914)276:37<34537:GIWTYQ>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
VESICLE TRANSPORT PATHWAYS; SACCHAROMYCES-CEREVISIAE; V-SNARE; PROTEIN-TRANSPORT; SYNTAXIN HOMOLOG; T-SNARE; ENDOPLASMIC-RETICULUM; VACUOLAR HYDROLASES; SECRETORY PATHWAY; SHUTTLE VECTORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: von Mollard, GF Univ Gottingen, Biochem Abt 2, Zentrum Biochem & Mol Zellbiol, Heinrich Duker Weg 12, D-37073 Gottingen, Germany Univ Gottingen Heinrich Duker Weg 12 Gottingen Germany D-37073
Citazione:
M. Dilcher et al., "Genetic interactions with the yeast Q-SNARE VTI1 reveal novel functions for the R-SNARE YKT6", J BIOL CHEM, 276(37), 2001, pp. 34537-34544

Abstract

SNARE proteins are required for fusion of transport vesicles with target membranes. Previously, we found that the yeast Q-SNARE Vti1p is involved in transport to the cis-Golgi, to the prevacuole/late endosome, and to the vacuole. Here we identified a previously uncharacterized gene, VTS1, and the R-SNARE YKT6 both as multicopy and as low copy suppressors of the growth andvacuolar transport defect in uti1-2 cells. Ykt6p was known to function in retrograde traffic to the cis-Golgi and homotypic vacuolar fusion. We foundthat VTI1 and YKT6 also interacted in traffic to the prevacuole and vacuole, indicating that these SNARE complexes contain Ykt6p, Vti1p, plus Pep12p and Ykt6p, Vti1p, Vam3p, plus Vam7p, respectively. As Ykt6p was required for several transport steps, R-SNAREs cannot be the sole determinants of specificity. To study the role of the 0 layer in the SNARE motif, we introducedthe mutations vti1-Q158R and ykt6-R165Q. SNARE complexes to which Ykt6p contributed a fourth glutamine residue in the 0 layer were nonfunctional, suggesting an essential function for arginine in the 0 layer of these complexes. vti1-Q158R cells had severe defects in several transport steps, indicating that the second arginine in the 0 layer interfered with function.

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Documento generato il 30/11/20 alle ore 03:33:06