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Titolo:
Augmented adriamycin sensitivity in cells transduced with an antisense tumor necrosis factor gene is mediated by caspase-3 downstream from reactive oxygen species
Autore:
Sasaki, M; Kobayashi, D; Watanabe, N;
Indirizzi:
Sapporo Med Univ, Sch Med, Div Lab Diag, Chuo Ku, Sapporo, Hokkaido 0600061, Japan Sapporo Med Univ Sapporo Hokkaido Japan 0600061 , Hokkaido 0600061, Japan Sapporo Med Univ, Sch Med, Dept Clin Lab Med, Chuo Ku, Sapporo, Hokkaido 0600061, Japan Sapporo Med Univ Sapporo Hokkaido Japan 0600061 , Hokkaido 0600061, Japan
Titolo Testata:
JAPANESE JOURNAL OF CANCER RESEARCH
fascicolo: 9, volume: 92, anno: 2001,
pagine: 983 - 988
SICI:
0910-5050(200109)92:9<983:AASICT>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PANCREATIC-CARCINOMA CELLS; ELEMENT-BINDING ACTIVITY; RESISTANT FACTOR; CANCER CELLS; APOPTOSIS; DOXORUBICIN; CYTOTOXICITY; ACTIVATION; ENHANCEMENT; CISPLATIN;
Keywords:
tumor necrosis factor; antisense; reactive oxygen species; caspase-3;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Watanabe, N Sapporo Med Univ, Sch Med, Div Lab Diag, Chuo Ku, South 1 West16, Sapporo, Hokkaido 0600061, Japan Sapporo Med Univ South 1 West 16 Sapporo Hokkaido Japan 0600061
Citazione:
M. Sasaki et al., "Augmented adriamycin sensitivity in cells transduced with an antisense tumor necrosis factor gene is mediated by caspase-3 downstream from reactive oxygen species", JPN J CANC, 92(9), 2001, pp. 983-988

Abstract

While transduction of an antisense tumor necrosis factor (TNF) gene sequence can augment the cytotoxicity of adriamycin (ADM) in human cancer cells, the specific effect of introducing this sequence on the signal transductionpathway leading to cell death remains unclear. In ADM-resistant pancreaticcarcinoma (PANC-1) cells, both the antioxidant N-acetyl-L-cysteine (NAC) and the caspase-3 inhibitor acetyl-L-aspartyl-L-Methionyl-L-glutaminyl-L-aspartyl-aldehyde (Ac-DMQD-CHO) prevented ADM-induced cytotoxicity. NAC additionally inhibited caspase-3 activity induced by ADM treatment, while Ac-DMQD-CHO showed no suppressive effect on reactive oxygen species (ROS). Stable antisense-TNF transfectants showed higher ADM sensitivity and greater ADM-induced ROS production and caspase-3 activity than mock transfectant or parent cells. These results indicate that increased caspase-3 activity downstream from ROS production is among the mechanisms by which transduction of theantisense TNF sequence of augments ADM sensitivity of pancreatic carcinomacells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:41:35