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Titolo:
The relevance of flow cytometry for biochemical analysis
Autore:
OConnor, JE; Callaghan, RC; Escudero, M; Herrera, G; Martinez, A; Monteiro, MDC; Montoliu, H;
Indirizzi:
Univ Valencia, Fac Med, Dept Bioquim & Biol Mol, Ctr Citometria, Valencia,Spain Univ Valencia Valencia Spain & Biol Mol, Ctr Citometria, Valencia,Spain Univ Valencia, Fac Med, Dept Patol, Valencia, Spain Univ Valencia Valencia Spain ncia, Fac Med, Dept Patol, Valencia, Spain Inst Super Ciencias Saude Norte, Dept Bioquim, Gandra PRD, Portugal Inst Super Ciencias Saude Norte Gandra PRD Portugal andra PRD, Portugal
Titolo Testata:
IUBMB LIFE
fascicolo: 4, volume: 51, anno: 2001,
pagine: 231 - 239
SICI:
1521-6543(200104)51:4<231:TROFCF>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE-LIVER MITOCHONDRIA; MULTIDRUG-RESISTANCE; FLUORESCENT DYES; LEUKEMIA-CELLS; LECTIN-BINDING; WHOLE-BLOOD; ASSAY; APOPTOSIS; CLASSIFICATION; HYBRIDIZATION;
Keywords:
cell function; cell sorting; flow cytometry; fluorescence; fluorochromes; isolated subcellular elements; multiplexed assay;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: O'Connor, JE Univ Valencia, Fac Med, Dept Bioquim & Biol Mol, Ctr Citometria, Valencia,Spain Univ Valencia Valencia Spain tr Citometria, Valencia,Spain
Citazione:
J.E. O'Connor et al., "The relevance of flow cytometry for biochemical analysis", IUBMB LIFE, 51(4), 2001, pp. 231-239

Abstract

Flow cytometry (FCM) allows the simultaneous measurement of multiple fluorescences and light scatter induced by illumination of single cells or microscopic particles in suspension, as they flow rapidly through a sensing area. In some systems, individual cells or particles may be sorted according tothe properties exhibited. By using appropriate fluorescent markers, FCM isunique in that multiple structural and functional parameters can be quantified simultaneously on a single-particle basis, whereas up to thousands of biological particles per second may be examined. FCM is increasingly used for basic, clinical, biotechnological, and environmental studies of biochemical relevance. In this critical review, we summarize the main advantages and limitations of FCM for biochemical studies and discuss briefly the most relevant parameters and analytical strategies. Graphical examples of the biological information provided by multiparametric FCM are presented. Also, this review contains specific sections on flow cytoenzymology, FCM analysis of isolated subcellular organelles, and cell-free FCM.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 13:33:25