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Titolo:
Hydrogenothermus marinus gen. nov., sp nov., a novel thermophilic hydrogen-oxidizing bacterium, recognition of Calderobacterium hydrogenophilum as a member of the genus Hydrogenobacter and proposal of the reclassification ofHydrogenobacter acidophilus as Hydrogenobaculum acidophilum gen. nov., comb. nov., in the phylum 'Hydrogenobacter/Aquifex'
Autore:
Stohr, R; Waberski, A; Volker, H; Tindall, BJ; Thomm, M;
Indirizzi:
Inst Allgemeine Mikrobiol, D-24118 Kiel, Germany Inst Allgemeine Mikrobiol Kiel Germany D-24118 ol, D-24118 Kiel, Germany DSMZ Braunschweig, D-38124 Braunschweig, Germany DSMZ Braunschweig Braunschweig Germany D-38124 124 Braunschweig, Germany
Titolo Testata:
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
, volume: 51, anno: 2001,
parte:, 5
pagine: 1853 - 1862
SICI:
1466-5026(200109)51:<1853:HMGNSN>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
YELLOWSTONE-NATIONAL-PARK; PHYLOGENETIC ANALYSIS; AQUIFEX-PYROPHILUS; LIPID-COMPOSITION; ELEMENTAL SULFUR; 2-METHYLTHIO-1,4-NAPHTHOQUINONE; QUINONE; GROWTH;
Keywords:
hydrogen oxidation; thermophilic bacteria; Aquifex; Hydrogenobacter;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Thomm, M Inst Allgemeine Mikrobiol, Bot Garten 1-9, D-24118 Kiel, Germany Inst Allgemeine Mikrobiol Bot Garten 1-9 Kiel Germany D-24118 ny
Citazione:
R. Stohr et al., "Hydrogenothermus marinus gen. nov., sp nov., a novel thermophilic hydrogen-oxidizing bacterium, recognition of Calderobacterium hydrogenophilum as a member of the genus Hydrogenobacter and proposal of the reclassification ofHydrogenobacter acidophilus as Hydrogenobaculum acidophilum gen. nov., comb. nov., in the phylum 'Hydrogenobacter/Aquifex'", INT J SY EV, 51, 2001, pp. 1853-1862

Abstract

A novel thermophilic, hydrogen-oxidizing bacterium, VM1(T), has been isolated from a marine hydrothermal area of Vulcano Island, Italy. Cells of the strain were Gram-negative rods, 2-4 mum long and 1-1.5 mum wide with four to seven monopolarly inserted flagella. Cells grew chemolithoautotrophicallyunder an atmosphere of H-2/CO2 (80:20) in the presence of low concentrations of O-2 (optimum 1-2%). Carbohydrates and peptide substrates were not utilized, neither for energy generation nor as a source of cellular carbon. Growth of VM1(T) occurred between 45 and 80 degreesC with an optimum at 65 degreesC. Growth was observed between pH 5 and 7. NaCl stimulated growth in the range 0.5-6% with an optimum at 2-3%. Hydrogen could not be replaced by elemental sulfur or thiosulfate as electron donors. Nitrate and sulfate were not used as electron acceptors. The major respiratory lipoquinone was a new menathioquinone. Analysis of the fatty acids of VM1(T) revealed straight-chain saturated C-18:0 and the unsaturated C(18:1)omega 9c and C(20:1)omega 9c as major components. The G+C content of the total DNA was 43 mol%. Phylogenetic analysis placed strain VM1(T) near the members of the genera Hydrogenobacter, Thermocrinis and Aquifex on a separate deep-branching phylogenetic lineage. Therefore, it is proposed that strain VM1(T) (= DSM 12046(T) = JCM 10974(T)) represents a novel species within a new genus, for which the name Hydrogenothermus marinus gen. nov., Sp. nov., is proposed. In addition, it is shown that Calderobacterium hydrogenophilum should be transferredto the genus Hydrogenobacter; the name Hydrogenobacter hydrogenophilus comb. nov. (DSM 2913(T) = JCM 8158(T)) is proposed for this organism. Furthermore, on the basis of 16S rRNA sequence analysis, Hydrogenobacter acidophilus is only distantly related to Hydrogenobacter species. Owing to this finding and its growth at low pH, the name Hydrogenobaculum acidophilum gen. nov., comb. nov., is proposed for Hydrogenobacter acidophilus. The type strainis JCM 8795(T) (= DSM 11251(T)).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 22:50:55