Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Induction of cell cycle arrest and apoptosis in human breast cancer cells by quercetin
Autore:
Choi, JA; Kim, JY; Lee, JY; Kang, CM; Kwon, HJ; Yoo, YD; Kim, TW; Lee, YS; Lee, SJ;
Indirizzi:
Korea Canc Ctr Hosp, Lab Radiat Effect, Nowon Ku, Seoul 139706, South Korea Korea Canc Ctr Hosp Seoul South Korea 139706 , Seoul 139706, South Korea Ajou Univ, Dept Biol Sci, Suwon 442749, South Korea Ajou Univ Suwon South Korea 442749 t Biol Sci, Suwon 442749, South Korea Sejong Univ, Dept Biosci & Biotechnol, Seoul 143747, South Korea Sejong Univ Seoul South Korea 143747 otechnol, Seoul 143747, South Korea
Titolo Testata:
INTERNATIONAL JOURNAL OF ONCOLOGY
fascicolo: 4, volume: 19, anno: 2001,
pagine: 837 - 844
SICI:
1019-6439(200110)19:4<837:IOCCAA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; HEAT-SHOCK PROTEIN; DNA-DAMAGE; DEPENDENT KINASES; CARCINOMA-CELLS; INHIBITORS; G(1); TRANSITION; PHOSPHORYLATION; FLAVONOIDS;
Keywords:
quercetin; G2/M arrest; p21(CIP1/WAF1); apoptosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, SJ Korea Canc Ctr Hosp, Lab Radiat Effect, Nowon Ku, Seoul 139706, South Korea Korea Canc Ctr Hosp Seoul South Korea 139706 139706, South Korea
Citazione:
J.A. Choi et al., "Induction of cell cycle arrest and apoptosis in human breast cancer cells by quercetin", INT J ONCOL, 19(4), 2001, pp. 837-844

Abstract

Quercetin, a widely distributed bioflavonoid, has been shown to induce growth inhibition in certain cancer cell types. In the present study we have pursued the mechanism of growth inhibition in MCF-7 human breast cancer cells. Quercetin treatment resulted in the accumulation of cells specifically at G2/M phase of the cell cycle. Mitotic index measured by MPM2 staining clearly showed that cells were transiently accumulated in M phase, 24 h after treatment. The transient M phase accumulation was accompanied by a transient increase in the levels of cyclin B1 and Cdc2 kinase activity. However, 24h or longer treatment caused a marked accumulation of cells in G2 instead of M phase. Levels of cyclin B1 and cyclin BI-associated Cdc2 kinase activity were also decreased. We also found that quercetin markedly increased Cdk-inhibitor p21(CIP1/WAF1) protein level after treatment for 48 h or longer,and the induction of p21(CIP1/WAF1) increased its association with Cdc2-cyclin B1 complex, however, up-regulation of p53 by quercetin was not observed. Quercetin also induced significant apoptosis in MCF-7 cells in addition to cell cycle arrest, and the induction of apoptosis was markedly blocked by antisense p21(CIP1/WAF1) expression. The present data, therefore, demonstrate that a flavonoid quercetin induces growth inhibition in the human breast carcinoma cell line MCF-7 through at least two different mechanisms; by inhibiting cell cycle progression through transient M phase accumulation and subsequent G2 arrest, and by inducing apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 13:23:52