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Titolo:
Human stomach-specific gene, CA11, is down-regulated in gastric cancer
Autore:
Shiozaki, K; Nakamori, S; Tsujie, M; Okami, J; Yamamoto, H; Nagano, H; Dono, K; Umeshita, K; Sakon, M; Furukawa, H; Hiratsuka, M; Kasugai, T; Ishiguro, S; Monden, M;
Indirizzi:
Osaka Univ, Grad Sch Med, Dept Surg & Clin Oncol, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 n Oncol, Suita, Osaka 5650871, Japan Osaka Med Ctr Canc & Cardiovasc Dis, Dept Surg, Osaka 5378511, Japan OsakaMed Ctr Canc & Cardiovasc Dis Osaka Japan 5378511 a 5378511, Japan Osaka Med Ctr Canc & Cardiovasc Dis, Dept Pathol, Osaka 5378511, Japan Osaka Med Ctr Canc & Cardiovasc Dis Osaka Japan 5378511 a 5378511, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF ONCOLOGY
fascicolo: 4, volume: 19, anno: 2001,
pagine: 701 - 707
SICI:
1019-6439(200110)19:4<701:HSGCID>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-LINES; COLORECTAL-CARCINOMA; FREQUENT LOSS; HETEROZYGOSITY; ADENOCARCINOMA; SUPPRESSOR; EXPRESSION;
Keywords:
gastric cancer; stomach-specific gene; gene expression; down-regulation; colony formation assay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Nakamori, S Osaka Univ, Grad Sch Med, Dept Surg & Clin Oncol, 2-2 Yamadaoka,E2, Suita,Osaka 5650871, Japan Osaka Univ 2-2 Yamadaoka,E2 Suita Osaka Japan 5650871 1, Japan
Citazione:
K. Shiozaki et al., "Human stomach-specific gene, CA11, is down-regulated in gastric cancer", INT J ONCOL, 19(4), 2001, pp. 701-707

Abstract

To reveal the implication in gastric cancer pathogenesis of the novel human gene referred to as CA11, which was recently isolated by a differential display technique using normal gastric mucosa and gastric cancer tissue, we examined CA11 expression in 50 primary gastric cancers and also introduced the CA11 gene into gastric cancer cells. RNA dot blot analysis against various human organs and developmental stages demonstrated that CA11 was intensively expressed especially in normal stomach tissue. Northern blot analysisshowed that expression of the CA11 gene in cancer tissue was down-regulated compared with normal tissue. Semi-quantitative RT-PCR also demonstrated that CA11 gene expression was decreased in 41 out of 50 (82%) of the gastriccancer tissues, when compared with normal stomach tissues, while no relationship was found between CA11 expression and various clinicopathological characteristics including histological type, depth of invasion, lymph node metastasis, and clinical stage. Immunohistochemical analysis with anti CA11 antibody showed that CA11-positive staining was observed in the surface regions of normal gastric epithelium, but was found faintly or not at all in cancer tissues. CA11 transfected MKN28 cells also displayed a marked decreasein the number of colony formations when compared to double normal controls. These findings suggest that the loss of CA11 expression in gastric tissues may play an important role in gastric carcinogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/06/20 alle ore 01:44:24