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Titolo:
Molecular analysis of p53 and K-ras in lung carcinomas of coal miners
Autore:
Sarkar, FH; Li, YW; Vallyathan, V;
Indirizzi:
Wayne State Univ, Sch Med, Dept Pathol, Karmanos Canc Inst, Detroit, MI 48201 USA Wayne State Univ Detroit MI USA 48201 os Canc Inst, Detroit, MI 48201 USA NIOSH, Hlth Effects Lab Div, Morgantown, WV USA NIOSH Morgantown WV USANIOSH, Hlth Effects Lab Div, Morgantown, WV USA
Titolo Testata:
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
fascicolo: 4, volume: 8, anno: 2001,
pagine: 453 - 459
SICI:
1107-3756(200110)8:4<453:MAOPAK>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
SQUAMOUS-CELL CARCINOMA; UNITED-STATES; SKIN-CANCER; MUTATIONS; GENE; MORTALITY; TUMORS; RADON; PNEUMOCONIOSIS; ADENOCARCINOMA;
Keywords:
lung cancer; coal workers' pneumoconiosis; p53 and K-ras genes; cigarette smoking; mutational spectrum; etiologic markers;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Sarkar, FH Wayne State Univ, Sch Med, Dept Pathol, Karmanos Canc Inst, 9374 Scott Hall,540 E Canfield Ave, Detroit, MI 48201 USA Wayne State Univ 9374 Scott Hall,540 E Canfield Ave Detroit MI USA 48201
Citazione:
F.H. Sarkar et al., "Molecular analysis of p53 and K-ras in lung carcinomas of coal miners", INT J MOL M, 8(4), 2001, pp. 453-459

Abstract

Thirty-three cases of non-small cell lung cancers (NSCLC) front the archives or National Coal Workers' Autopsy Study were studied for mutational alterations in p53 and K-ras using PCR-SSCP, DNA sequencing and PCR-oligonucleotide probe hybridization techniques. Mutations of the p53 were observed in 4 smokers (19%) and one in a never smoker (8%). Two polymorphisms in smokers were detected at codon 213, a common site for sequence variation. Among the smokers the p53 mutations were in the heavy smokers. In never smokers there was only a single p53 mutation and two K-ras mutations. In never smokers the frequency of K-ras mutations was similar (17%) in smokers, but one never smoker had two K-ras mutations. Mutations of p53 were more frequent in adenocarcinomas (27%) and they were AT-GC transitions. Four of It (36%) adenocarcinomas were found to have K-ras codon 12 mutations, and one adenocarcinoma had two K-ras mutations. There were two large cell undifferentiated carcinomas with p53 mutation and one with a K-ras mutation. Two of the 16 squamous cell carcinomas were positive for p53 mutation, while no K-ras mutations were found in this group. The results of these preliminary studies indicate a moderately different mutational spectrum of p53 and K-ras in coal miners independent of cigarette smoking. The mutational spectrum observed inthis study of coal miners with heavy cigarette smoking history suggest a protective effect of coal mine dust in preventing abnormal mutations inducedby chemical carcinogens in cigarette smoke or reactive oxygen species. These limited preliminary studies provide insight into the possibility of accurately measuring changes in etiologic markers to unravel the uncertainties of cancer in coal miners.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 11:48:59