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Titolo:
Retroviral vectors bearing IgG-binding motifs for antibody-mediated targeting of vascular endothelial growth factor receptors
Autore:
Masood, R; Gordon, EM; Whitley, MD; Wu, BW; Cannon, P; Evans, L; Anderson, WF; Gill, P; Hall, FL;
Indirizzi:
Univ So Calif, Sch Med, Gene Therapy Labs, Los Angeles, CA 90033 USA Univ So Calif Los Angeles CA USA 90033 py Labs, Los Angeles, CA 90033 USA Univ So Calif, Sch Med, Dept Pediat, Los Angeles, CA 90033 USA Univ So Calif Los Angeles CA USA 90033 Pediat, Los Angeles, CA 90033 USA Univ So Calif, Sch Med, Dept Surg, Los Angeles, CA 90033 USA Univ So Calif Los Angeles CA USA 90033 pt Surg, Los Angeles, CA 90033 USA Univ So Calif, Sch Med, Dept Biochem, Los Angeles, CA 90033 USA Univ So Calif Los Angeles CA USA 90033 Biochem, Los Angeles, CA 90033 USA Univ So Calif, Sch Med, Dept Mol Pharmacol Toxicol, Los Angeles, CA 90033 USA Univ So Calif Los Angeles CA USA 90033 Toxicol, Los Angeles, CA 90033 USA Univ So Calif, Sch Med, Dept Med, Los Angeles, CA 90033 USA Univ So CalifLos Angeles CA USA 90033 ept Med, Los Angeles, CA 90033 USA NIAID, Rocky Mt Labs, Persistent Viral Dis Lab, Hamilton, MT 59840 USA NIAID Hamilton MT USA 59840 sistent Viral Dis Lab, Hamilton, MT 59840 USA
Titolo Testata:
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
fascicolo: 4, volume: 8, anno: 2001,
pagine: 335 - 343
SICI:
1107-3756(200110)8:4<335:RVBIMF>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
STAPHYLOCOCCAL PROTEIN-A; ENVELOPE PROTEIN; VIRUS VECTORS; GENE-TRANSFER; THERAPY; SYSTEM; GLYCOPROTEINS; DELIVERY; FRAGMENT; COMPLEX;
Keywords:
retroviral vectors; vascular endothelial growth factor receptors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Hall, FL USC, Gene Therapy Labs, 1975 Zonal Ave,KAM30, Los Angeles, CA 90089 USA USC 1975 Zonal Ave,KAM30 Los Angeles CA USA 90089 s, CA 90089 USA
Citazione:
R. Masood et al., "Retroviral vectors bearing IgG-binding motifs for antibody-mediated targeting of vascular endothelial growth factor receptors", INT J MOL M, 8(4), 2001, pp. 335-343

Abstract

Targeting retroviral vectors to tumor vasculature is an important goal of cancer gene therapy. In this study, we report a novel targeting approach wherein IgG-binding peptides were inserted into the Moloney murine leukemia virus (MuLV) envelope (env) protein. The modifications on the viral env included replacement of the entire receptor binding region of the viral env with protein A (or ZZ) domains. The truncated env incorporating IgG-binding motifs (known as 'escort' proteins) provided the targeting function, while the coexpressed wild-type (WT) env protein enabled viral fusion and cell entry. An anti-human VEGF receptor (Flk-1/KDR) antibody served as a molecular bridge, directing the retroviral vector to the endothelial cell. Hence, the IgG-targeted vectors bound to the Flk-1/KDR antibody which in turn bound toVEGF receptors on Kaposi sarcoma, KSY1, endothelial cells. The net effect was increased viral fusion and infectivity of IgG-bound retroviral vectors when compared to non-targeted vectors bearing WT env alone. These data provide the proof of concept that IgG-binding vector/VEGF receptor antibody complexes may be used to enhance retroviral gene delivery to activated endothelial cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 14:06:50