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Titolo:
CMV-driven expression of green fluorescent protein (GFP) in male germ cells of transgenic mice and its effect on fertility
Autore:
Villuendas, G; Gutierrez-Adan, A; Jimenez, A; Rojo, C; Roldan, ERS; Pintado, B;
Indirizzi:
INIA, Dept Reprod Anim & CRZ, Madrid 28040, Spain INIA Madrid Spain 28040 NIA, Dept Reprod Anim & CRZ, Madrid 28040, Spain CSIC, Museo Nacl Ciencias Nat, E-28006 Madrid, Spain CSIC Madrid Spain E-28006 Museo Nacl Ciencias Nat, E-28006 Madrid, Spain
Titolo Testata:
INTERNATIONAL JOURNAL OF ANDROLOGY
fascicolo: 5, volume: 24, anno: 2001,
pagine: 300 - 305
SICI:
0105-6263(200110)24:5<300:CEOGFP>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERMEDIATE FILAMENTS; SPERMATOGENIC CELLS; SERTOLI CELLS; MOUSE;
Keywords:
GFP expression; male germ cells; transgenic mice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Gutierrez-Adan, A INIA, Dept Reprod Anim & CRZ, Ctra Coruna Km 5,9, Madrid28040, Spain INIA Ctra Coruna Km 5,9 Madrid Spain 28040 28040, Spain
Citazione:
G. Villuendas et al., "CMV-driven expression of green fluorescent protein (GFP) in male germ cells of transgenic mice and its effect on fertility", INT J ANDR, 24(5), 2001, pp. 300-305

Abstract

To determine if the expression of green fluorescent protein (GFP) during spermatogenesis can compromise the fertility of transgenic animals, we have produced mouse transgenic lines expressing GFP in the testis under the control of the potent immediate early promoter and enhancer region of the humancytomegalovirus (CMV). GFP expression was detected in the germ cells with no apparent effect on the fertility of any of the five transgenic lines studied. We demonstrate that the CMV promoter is transcriptionally active in the testes of mice aged 7 days. However, protein could be visually detected only after day 10. GFP was not found either in mature spermatozoa or in Sertoli cells, but it was evident in round spermatids from seminiferous tubules and in cytoplasmic drops of spermatozoa from the epididymis. We also detected GFP in spermatogonia expressing c-kit, which indicates that GFP expression driven by the CMV promoter takes place during the proliferative phase of spermatogenesis. The expression of GFP during spermatogenesis did not affect the fertility of transgenic mice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 08:57:54