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Titolo:
Activity of testis angiotensin converting enzyme (ACE) in ejaculated humanspermatozoa
Autore:
Shibahara, H; Kamata, M; Hu, J; Nakagawa, H; Obara, H; Kondoh, N; Shima, H; Sato, I;
Indirizzi:
Jichi Med Sch, Dept Obstet & Gynecol, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan BML Inc, R&D Ctr, Kawagoe, Saitama, Japan BML Inc Kawagoe Saitama JapanBML Inc, R&D Ctr, Kawagoe, Saitama, Japan Hyogo Med Univ, Dept Urol, Nishinomiya, Hyogo, Japan Hyogo Med Univ Nishinomiya Hyogo Japan t Urol, Nishinomiya, Hyogo, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF ANDROLOGY
fascicolo: 5, volume: 24, anno: 2001,
pagine: 295 - 299
SICI:
0105-6263(200110)24:5<295:AOTACE>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-PRESSURE; MALE-FERTILITY; LOCALIZATION; EXPRESSION; SPERM; RAT; CAPACITATION; EPIDIDYMIS; MOTILITY; TISSUES;
Keywords:
human sperm; membrane protein; sperm motility; testis ACE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Shibahara, H Jichi Med Sch, Dept Obstet & Gynecol, 3311-1 Yakushiji, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch 3311-1 Yakushiji Minami Kawachi Tochigi Japan 3290498
Citazione:
H. Shibahara et al., "Activity of testis angiotensin converting enzyme (ACE) in ejaculated humanspermatozoa", INT J ANDR, 24(5), 2001, pp. 295-299

Abstract

Testicular angiotensin converting enzyme (ACE) isozyme is likely to play important functional roles in male reproduction. Several studies have shown that ACE is released from human spermatozoa during capacitation and that ACE is associated with reduced sperm motility. Recently, we established an assay to detect testicular ACE activity in human spermatozoa. The purpose of this study was to determine if testicular ACE activity is related to sperm motility in human ejaculates. Semen samples were collected from 80 infertile patients. According to the semen characteristics, they were divided into four (WHO) categories. Enzyme activities of ACE in spermatozoa (testicular ACE) and seminal plasma (somatic ACE) were spectrophotometrically determined. Total testicular ACE activity in spermatozoa was measured by solubilization of spermatozoa with Triton X-100. Membrane testicular ACE activity was measured in a sperm : PBS suspension. Sperm concentration and sperm motility were 136.6 +/- 154.1 x 10(6)/mL and 58.6 +/- 23.4%, respectively (mean SID). Enzyme activities of membrane testicular ACE, total testicular ACE and somatic ACE were 0.273 +/- 1.219 muU/10(6) spermatozoa, 0.35 +/- 1.34 muU/10(6) spermatozoa and 684.7 +/- 226.6 mU/mL, respectively. A negative correlation was observed between sperm motility and membrane testicular ACE activity (p < 0.05). Membrane testicular ACE activity in 44 normal semen sampleswas 0.04 <plus/minus>0.02 muU/10(6) spermatozoa, whilst that in 36 abnormal semen samples was 0.24 +/- 0.42 muU/10(6) spermatozoa. There was a significant difference between these two groups (p < 0.01). Membrane testicular ACE in sperm samples from normozoospermic men was significantly lower than that from oligoasthenozoospermic men (P < 0.05). These findings suggest thattesticular ACE is released from normal functional spermatozoa for them to have fertilizing ability.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 10:31:38