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Titolo:
Structural organization of the rat mitogen-activated protein kinase phosphatase 2 gene
Autore:
Zhang, T; Choy, M; Jo, M; Roberson, MS;
Indirizzi:
Cornell Univ, Coll Vet Med, Dept Biomed Sci, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 d, Dept Biomed Sci, Ithaca, NY 14853 USA
Titolo Testata:
GENE
fascicolo: 1, volume: 273, anno: 2001,
pagine: 71 - 79
SICI:
0378-1119(20010725)273:1<71:SOOTRM>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOCHROME-P-450 OXIDOREDUCTASE GENE; HORMONE RECEPTOR GENE; TATA-LESS PROMOTER; TRANSCRIPTIONAL REGULATION; TYROSINE-PHOSPHATASE; SIGNALING PATHWAY; EXPRESSION; CELLS; MKP-1; APOPTOSIS;
Keywords:
dual specificity protein phosphatase; mitogen-activated protein kinase; TATA-less promoter; transcription start site;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Roberson, MS Cornell Univ, Coll Vet Med, Dept Biomed Sci, Ithaca, NY 14853USA Cornell Univ Ithaca NY USA 14853 d Sci, Ithaca, NY 14853 USA
Citazione:
T. Zhang et al., "Structural organization of the rat mitogen-activated protein kinase phosphatase 2 gene", GENE, 273(1), 2001, pp. 71-79

Abstract

Mitogen-activated protein kinase (MAPK) phosphatases (MKPs) are dual specificity protein phosphatases that specifically inactivate MAPKs. Regulated expression of MKPs plays a key role in determining their physiological function. However, little is known about the molecular mechanism of the activation of MKP genes. In this study, we cloned the rat MKP-2 gene and characterized its structure. The MKP-2 gene has four exons and three introns. The organization of exons of the MKP-2 gene is very similar to that of the MKP-1 gene, suggesting that MKP-1 and MKP-2 are derived from the same ancestral gene. We identified multiple transcription start sites (TSSs) for the MKP-2 gene. There is no functional TATA motif in the 5' proximal region of the TSSs. Instead, this region is highly GC-rich and has two putative Sp1 sites. A1.8 kb 5' flanking region of the MKP-2 gene is sufficient to mediate transcriptional activation of the luciferase reporter gene by phorbol ester in GH3 cells, These results provide essential information about structural organization and regulatory sequences of the MKP-2 gene for further investigation of the molecular mechanisms of MKP-2 induction by extracellular stimuli. (C) 2001 Published by Elsevier Science B.V. All rights reserved.

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Documento generato il 08/04/20 alle ore 12:09:57