Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Differential expression and subcellular distribution of the mouse metastasis-associated proteins Mta1 and Mta3
Autore:
Simpson, A; Uitto, J; Rodeck, U; Mahoney, MG;
Indirizzi:
Jefferson Med Coll, Dept Dermatol & Cutaneous Biol, Philadelphia, PA 19107USA Jefferson Med Coll Philadelphia PA USA 19107 l, Philadelphia, PA 19107USA Thomas Jefferson Univ, Jefferson Inst Mol Med, Dept Biochem & Mol Pharmacol, Philadelphia, PA 19107 USA Thomas Jefferson Univ Philadelphia PA USA 19107 hiladelphia, PA 19107 USA
Titolo Testata:
GENE
fascicolo: 1, volume: 273, anno: 2001,
pagine: 29 - 39
SICI:
0378-1119(20010725)273:1<29:DEASDO>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HISTONE DEACETYLASE; PHOSPHATIDYLINOSITOL 3-KINASE; MAMMARY ADENOCARCINOMA; TYROSINE KINASE; ADAPTER PROTEIN; GENE; COMPLEX; GRB2; OVEREXPRESSION; SEQUENCE;
Keywords:
Fyn; Grb2; nucleosome-remodeling histone-deacetylase complex; Ste homology 3 binding domain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Mahoney, MG Jefferson Med Coll, Dept Dermatol & Cutaneous Biol, 233 S 10thSt,Room 419BLSB, Philadelphia, PA 19107 USA Jefferson Med Coll 233 S 10th St,Room 419 BLSB Philadelphia PA USA 19107
Citazione:
A. Simpson et al., "Differential expression and subcellular distribution of the mouse metastasis-associated proteins Mta1 and Mta3", GENE, 273(1), 2001, pp. 29-39

Abstract

The human metastasis-associated gene (MTA1) is overexpressed in cell linesand tissues representing metastatic tumors. Here we report cloning of the mouse Mta1 as well as a novel structurally related mouse gene, Mta3. The mouse Mta1 protein shares 94 and 59% homology to the human MTA1 and mouse Mta3 proteins, respectively. Northern blotting analysis using an Mta1 cDNA probe revealed a prevalent 3 kb hybridization signal in all mouse tissues except the skeletal muscle while a smaller similar to 1.0 kb mRNA product was also detected in the heart. Mta3 transcripts (similar to2 kb) were detected in most tissues with an additional similar to6.2 kb signal detected in the brain. In vitro transcription/ translation of the full-length Mta1 and Mta3cDNAs generated products of the expected molecular masses, i.e. 80 and 60 kDa, respectively. To assess subcellular localization, green fluorescence protein (GFP)-tagged expression constructs of Mta1 and Mta3 and various deletion constructs of GFP-Mta1 were transiently expressed in Balb/MK keratinocytes. GFP-Mta1 was found exclusively in the nucleus while GFP-Mta3 was present in both the nucleus and cytoplasm. Compared to Mta3, the carboxy terminal end of Mta1 contains an additional nuclear localization signal (NLS) anda proline-rich Src homology 3 (SH3) ligand. The results of transient expression experiments of various Mta1 fragments containing these domains in different combinations indicated that nuclear localization of Mta1 depended onthe presence of at least one NLS and one SH3 binding site. These SH3 ligands appeared to be functional as they facilitated interaction with the adaptor protein, Grb2, and the Src-family tyrosine kinase, Fyn. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 08:45:36