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Titolo:
Molecular cloning of natriuretic peptide receptor A from bullfrog (Rana catesbeiana) brain and its functional expression
Autore:
Sekiguchi, T; Miyamoto, K; Mizutani, T; Yamada, K; Yazawa, T; Yoshino, M; Minegishi, T; Takei, Y; Kangawa, K; Minamino, N; Saito, Y; Kojima, M;
Indirizzi:
Fukui Med Univ, Dept Biochem, Matsuoka, Fukui 9101193, Japan Fukui Med Univ Matsuoka Fukui Japan 9101193 atsuoka, Fukui 9101193, Japan JST, CREST, Tokyo 1020081, Japan JST Tokyo Japan 1020081JST, CREST, Tokyo 1020081, Japan Gunma Univ, Sch Med, Dept Obstet & Gynecol, Maebashi, Gumma 3718511, JapanGunma Univ Maebashi Gumma Japan 3718511 l, Maebashi, Gumma 3718511, Japan Univ Tokyo, Ocean Res Inst, Physiol Lab, Tokyo 1648639, Japan Univ Tokyo Tokyo Japan 1648639 s Inst, Physiol Lab, Tokyo 1648639, Japan Natl Cardiovasc Ctr, Res Inst, Dept Biochem, Suita, Osaka 5658565, Japan Natl Cardiovasc Ctr Suita Osaka Japan 5658565 Suita, Osaka 5658565, Japan
Titolo Testata:
GENE
fascicolo: 2, volume: 273, anno: 2001,
pagine: 251 - 257
SICI:
0378-1119(20010808)273:2<251:MCONPR>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
RELATIVE BIOLOGICAL-ACTIVITY; AMINO-ACID-SEQUENCE; SIGNAL TRANSDUCTION; GUANYLATE-CYCLASE; PORCINE BRAIN; FROG-HEART; ATRIAL; EEL; IDENTIFICATION; ACTIVATION;
Keywords:
bullfrog brain; natriuretic peptide receptor A; cDNA cloning; functional expression; cyclic guanosine 3 ' 5 '-monophosphate production; Northern blotting;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Miyamoto, K Fukui Med Univ, Dept Biochem, Shimoaizuki, Matsuoka, Fukui 9101193, Japan Fukui Med Univ Shimoaizuki Matsuoka Fukui Japan 9101193 Japan
Citazione:
T. Sekiguchi et al., "Molecular cloning of natriuretic peptide receptor A from bullfrog (Rana catesbeiana) brain and its functional expression", GENE, 273(2), 2001, pp. 251-257

Abstract

A comparative study of natriuretic peptide receptor (NPR) was performed bycloning the NPR-A receptor subtype from the bullfrog (Rana catesbeiana) brain and analyzing its functional expression. Like other mammalian NPR-A receptors, the bullfrog NPR-A receptor consists of an extracellular ligand binding domain, a hydrophobic transmembrane domain, a kinase-like domain and aguanylate cyclase domain. Sequence comparison among the bullfrog and mammalian receptors revealed a relatively low (approximate to 45%) similarity inthe extracellular domain compared to a very high similarity (approximate to 92%) in the cytoplasmic regulatory and catalytic domains. Expression of NPR-A mRNA was detected in various bullfrog tissues including the brain, heart, lung, kidney and liver; highest levels were observed in lung. Functional expression of the receptor in COS-7 cells revealed that frog atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) elicited cyclic guanosine 3 '5 ' -monophosphate production by stimulating the receptor in a dose-dependent manner from 10(-10) M concentrations. Rat ANP was also effective in stimulating the frog receptor whereas rat BNP and porcine BNP were less responsive to the receptor. On the other hand, frog C-type natriuretic peptide (CNP) as well as porcine CNP stimulated the receptor only at high concentrations (10(-7) M). This clearly indicates that the bullfrog receptoris a counterpart of mammalian NPR-A, and is specific for ANP or BNP but not for CNP. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:33:42