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Titolo:
Rho family GTPases regulate VEGF-stimulated endothelial cell motility
Autore:
Soga, N; Namba, N; McAllister, S; Cornelius, L; Teitelbaum, SL; Dowdy, SF; Kawamura, J; Hruska, KA;
Indirizzi:
Washington Univ, Sch Med, Dept Med, Div Renal, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 , Div Renal, St Louis, MO 63110 USA Washington Univ, Sch Med, Dept Med, Div Dermatol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 iv Dermatol, St Louis, MO 63110 USA Washington Univ, Sch Med, Dept Pathol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 Dept Pathol, St Louis, MO 63110 USA Washington Univ, Sch Med, Dept Cell Biol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 t Cell Biol, St Louis, MO 63110 USA Washington Univ, Sch Med, Howard Hughes Med Inst, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 es Med Inst, St Louis, MO 63110 USA Mie Univ, Sch Med, Dept Urol, Tsu, Mie 514, Japan Mie Univ Tsu Mie Japan 514 Univ, Sch Med, Dept Urol, Tsu, Mie 514, Japan
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 1, volume: 269, anno: 2001,
pagine: 73 - 87
SICI:
0014-4827(20010910)269:1<73:RFGRVE>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; TAT FUSION PROTEINS; GROWTH-FACTOR; FOCAL ADHESIONS; TYROSINE PHOSPHORYLATION; ALPHA(V)BETA(3) INTEGRIN; SIGNALING MECHANISMS; EXTRACELLULAR-MATRIX; ACTIN REORGANIZATION; FLK-1/KDR RECEPTOR;
Keywords:
Rac; rho; CDC42; endothelial cells; VEGF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: Hruska, KA Washington Univ, Sch Med, Dept Med, Div Renal, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 , St Louis, MO 63110 USA
Citazione:
N. Soga et al., "Rho family GTPases regulate VEGF-stimulated endothelial cell motility", EXP CELL RE, 269(1), 2001, pp. 73-87

Abstract

Migration of endothelial cells induced by vascular endothelial growth factor (VEGF) is a critical step in angiogenesis. Stimulation of motility by growth factors such as VEGF requires interaction with the signal transductionpathways activated by the extracellular matrix (ECM). Here we demonstrate that the Rac GTPase is the critical intersection activated by type 1 collagen ECM and VEGF during stimulation of endothelial cell motility. To analyzethe role of the Rho family GTPases in VEGF-stimulated endothelial cell chemotaxis and ECM-stimulated haptotaxis, we transduced the respective fusion proteins in human foreskin dermal endothelial cells using a Tat peptide from the human immunodeficiency virus Tat protein. VEGF signaling required Racactivation during chemotaxis, and Rac and Cdc42 were activated during haptotaxis on type I collagen. Similar to VEGF, Rac activation induced an increase in endothelial cell stress fiber and focal adhesion. Surprisingly, Rho activation was not present in collagen-induced haptotaxis or stimulation ofchemotaxis by VEGF, although Rho induced stress fibers and focal adhesionssimilar to Rac activation. The result of constitutive Rho activation was an inhibition of haptotaxis. Thus, Rac is required and sufficient for the activation of endothelial cell haptotaxis and VEGF-stimulated chemotaxis. (C)2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 04:24:07