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Titolo:
Variations in 5-fluorouracil concentrations of colorectal tissues as compared with dihydropyrimidine dehydrogenase (DPD) enzyme activities and DPD messenger RNA levels
Autore:
Tanaka-Nozaki, M; Onda, M; Tanaka, N; Kato, S;
Indirizzi:
Nippon Med Coll, Dept Surg 1, Bunkyo Ku, Tokyo 1138603, Japan Nippon Med Coll Tokyo Japan 1138603 g 1, Bunkyo Ku, Tokyo 1138603, Japan
Titolo Testata:
CLINICAL CANCER RESEARCH
fascicolo: 9, volume: 7, anno: 2001,
pagine: 2783 - 2787
SICI:
1078-0432(200109)7:9<2783:VI5COC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANCER-PATIENTS; DEFICIENCY; TOXICITY; INFUSION; PLASMA; TUMORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Tanaka-Nozaki, M Nippon Med Coll, Dept Surg 1, Bunkyo Ku, 1-1-5 Sendagi, Tokyo 1138603, Japan Nippon Med Coll 1-1-5 Sendagi Tokyo Japan 1138603 Japan
Citazione:
M. Tanaka-Nozaki et al., "Variations in 5-fluorouracil concentrations of colorectal tissues as compared with dihydropyrimidine dehydrogenase (DPD) enzyme activities and DPD messenger RNA levels", CLIN CANC R, 7(9), 2001, pp. 2783-2787

Abstract

Dihydropyrimidine dehydrogenase (DPD) is the initial key enzyme in 5-fluorouracil (5-FU) catabolism. We measured DPD activities represented as DPD protein levels (units/mg protein) and the associated mRNA levels in tumorous and normal tissues from 40 colorectal cancer patients, and we studied the relation to 5-FU concentrations in the same samples after treatment with doxifluridine, a prodrug of 5-FU. DPD mRNA levels were also measured in biopsysamples before treatment for comparison with those in surgical samples. 5-FU concentrations in tumors were higher than those in normal tissues (P < 0.05) and were inversely associated with DPD protein levels (r = -0.463; P <0.05). DPD activities in tumorous and normal tissues showed a significant correlation (r = 0.527; P < 0.01). DPD protein levels correlated with theirmRNA levels detected by semiquantitative reverse transcription-PCR in tumor tissues (r = 0.740; P < 0.01). DPD mRNA levels in tumor biopsy specimens correlated with those in surgical specimens (r =: 0.366; P < 0.05). These results suggest DPD activities in tumors to be predictive of 5-FU levels in colorectal cancer tissues and are reflected by DPD, mRNA levels as measuredby reverse transcription-PCR.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 13:52:47