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Titolo:
Targeted overexpression of CCAAT/enhancer-binding protein-delta evokes enhanced gene transcription of platelet-derived growth factor alpha-receptor in vascular smooth muscle cells
Autore:
Yang, ZH; Kitami, Y; Takata, Y; Okura, T; Hiwada, K;
Indirizzi:
Ehime Univ, Sch Med, Dept Internal Med 2, Matsuyama, Ehime 7910295, Japan Ehime Univ Matsuyama Ehime Japan 7910295 Matsuyama, Ehime 7910295, Japan
Titolo Testata:
CIRCULATION RESEARCH
fascicolo: 6, volume: 89, anno: 2001,
pagine: 503 - 508
SICI:
0009-7330(20010914)89:6<503:TOOCPE>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
3 C/EBP ISOFORMS; REGULATED EXPRESSION; TRANSGENIC MICE; LEUCINE ZIPPER; FAMILY; INDUCTION; ELEMENTS; STAT3;
Keywords:
platelet-derived growth factor alpha-receptor vascular smooth muscle cells; CCAAT/enhancer-binding protein-alpha; transgenic rats; gene expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Kitami, Y Ehime Univ, Sch Med, Dept Internal Med 2, Matsuyama, Ehime 7910295, Japan Ehime Univ Matsuyama Ehime Japan 7910295 , Ehime 7910295, Japan
Citazione:
Z.H. Yang et al., "Targeted overexpression of CCAAT/enhancer-binding protein-delta evokes enhanced gene transcription of platelet-derived growth factor alpha-receptor in vascular smooth muscle cells", CIRCUL RES, 89(6), 2001, pp. 503-508

Abstract

Platelet-derived growth factor (PDGF) is thought to play a significant role in various models of vascular remodeling, particularly in the early process of vascular diseases. Its action is mediated by its specific receptor, the PDGF receptor. The PDGF alpha -receptor (PDGF alphaR) plays an importantrole in the growth and proliferation of vascular smooth muscle cells (VSMCs), and its gene expression is thought to be regulated by several potentialtranscriptional nuclear factors. However, the detailed mechanisms of tissue-specific transactivation of the PDGFaR gene in VSMCs remain to be clarified. We have previously demonstrated that the rat PDGF alphaR gene contains an enhancer core sequence for CCAAT/enhancer-binding proteins (C/EBPs) in its promoter region, and we have also suggested that C/EBP-delta is the principal factor involved in the induction of tissue-specific transcriptional activity of the PDGFaR gene in VSMCs. To explore the definitive roles of C/EBP-delta protein on PDGFaR gene transcription in VSMCs, we developed C/EBP-delta transgenic rats by using a chimeric fusion gene of the mouse smooth muscle a-actin promoter and an entire coding region of rat C/EBP-delta cDNA. This report describes the first successful targeted overexpression of C/EBP-delta capable of inducing PDGFaR gene transcription and modifying cell proliferative activity to PDGFs. Targeted overexpression of C/EBP-delta evokes high levels of PDGFaR gene expression, susceptibility to VSMC growth, andproliferation of VSMCs to PDGFs. The results obtained reveal evidence of anew role and new functional significance of C/EBP-delta on VSMC growth viathe PDGFaR during the process of vascular remodeling and atherosclerosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 03:18:15