Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effect of kinase-negative EGFR gene on differentiation of embryonic germ cell line EG4
Autore:
Xu, X; Cong, XQ; Zhang, SQ; Yan, YH;
Indirizzi:
Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Cell Biol, Shanghai 200031,Peoples R China Chinese Acad Sci Shanghai Peoples R China 200031 200031,Peoples R China
Titolo Testata:
CHINESE SCIENCE BULLETIN
fascicolo: 16, volume: 46, anno: 2001,
pagine: 1364 -
SICI:
1001-6538(200108)46:16<1364:EOKEGO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR RECEPTORS; IN-VITRO; CARCINOMA-CELLS; STEM-CELLS; EXPRESSION; INSULIN; CULTURE;
Keywords:
EGFR; embryonic germ cell line; gene transfection; cell differentiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Yan, YH Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Cell Biol, Shanghai200031,Peoples R China Chinese Acad Sci Shanghai Peoples R China 200031 Peoples R China
Citazione:
X. Xu et al., "Effect of kinase-negative EGFR gene on differentiation of embryonic germ cell line EG4", CHIN SCI B, 46(16), 2001, pp. 1364

Abstract

EG4 cells derived from primordial germ cells (PGCs) of 10.5 d post coitum 129/svJ mouse embryos can be used as a model system for in vitro differentiation study due to their pluripotential development ability. ECA cell lineswith stable expression of kinase-negative EGFR cDNA, designated EG4-EGFR(d), were generated by gene transfection. We found that: (i) EG4-EGFR(d) cells share the similar morphology and growing character with wildtype cells that can maintain undifferentiated state in long term culture. (ii) Treatmentof EG4 cells with RA resulted in differentiation of adipocyte, while in mutant clones of EG4-EGFR(d), adipocytes were sparse or absent under the samecondition, Indicating the role of EGFR expressed during adipocyte development (iii) Histological analysis showed that predominant tissues in teratocarcinomas derived from EG4-EGFR(d) cells and wildtype cells are different. Alarge amount of undifferentiated cells was present in those coming from mutant cell clones. In addition some cardiac and skeletal muscles are prominently differentiated cell types. ECA wildtype cells produced multiple differentiated cell types of three primary germ layers such as cartilage, epithelia and neural tube. These studies suggested that EGFR-dependent differentiation was inhibited in kinase-negative EG4 cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 06:43:59