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Titolo:
Production of butyrylcholinesterase by Caco-2 cells: lack of relationship with triglyceride production
Autore:
Randell, EW; Rogers, B; Smith, E;
Indirizzi:
Hlth Sci Ctr, Fac Med, Dept Lab Med, St Johns, NF A1B 3V6, Canada Hlth SciCtr St Johns NF Canada A1B 3V6 Med, St Johns, NF A1B 3V6, Canada Mem Univ Newfoundland, Fac Sci, Dept Biochem, St Johns, NF A1C 5S7, CanadaMem Univ Newfoundland St Johns NF Canada A1C 5S7 ohns, NF A1C 5S7, Canada
Titolo Testata:
CELLULAR AND MOLECULAR LIFE SCIENCES
fascicolo: 9, volume: 58, anno: 2001,
pagine: 1327 - 1332
SICI:
1420-682X(200108)58:9<1327:POBBCC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIPOPROTEIN CHOLESTEROL RATIO; LOW-DENSITY LIPOPROTEIN; DIABETES-MELLITUS; LINE CACO-2; SERUM PSEUDOCHOLINESTERASE; APOLIPOPROTEIN-B; FATTY-ACID; CHOLINESTERASE; SECRETION; INSULIN;
Keywords:
butyrylcholinesterase; cholinesterase; obesity; lipid; triglyceride; insulin; Caco-2; diabetes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Randell, EW Hlth Sci Ctr, Fac Med, Dept Lab Med, 300 Prince Philip Dr, St Johns, NF A1B 3V6, Canada Hlth Sci Ctr 300 Prince Philip Dr St Johns NF Canada A1B 3V6 a
Citazione:
E.W. Randell et al., "Production of butyrylcholinesterase by Caco-2 cells: lack of relationship with triglyceride production", CELL MOL L, 58(9), 2001, pp. 1327-1332

Abstract

Elevated levels of butyrylcholinesterase activity occur under a number of hypertriglyceridemic conditions, including diabetes and obesity. This studyexamines whether butyrylcholinesterase activity has a direct effect on triglyceride production, using Caco-2 cells, a human intestinal adenocarcinomacell line. Caco-2 cells were incubated with 500 muM oleate to stimulate triglyceride production, and butyrylcholinesterase activity was measured in the cellular homogenate. Butyrylcholinesterase activity was approximately 3x10(-3) mu mol/min per milligram protein. Although triglyceride production increased by almost five-fold after 18 h of stimulation with oleate, butyrylcholinesterase activity was not increased. Furthermore, inhibition of butyrylcholinesterase activity using 1 mM tetraisopropylpyrophosphoramide did not significantly affect triglyceride production or secretion. Human insulin (100 muU/ml) increased the production of butyrylcholinesterase without increasing triglyceride production. This demonstrates that stimulation of fattyacid production and butyrylcholinesterase activity occur by independent mechanisms and suggests that their correlation in hyperlipidemic conditions is not due to a direct relationship in production in situ.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 08:34:44