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Titolo:
Non-steroidal anti-inflammatory drugs induce apoptosis in gastric cancer cells through up-regulation of bax and bak
Autore:
Zhou, XM; Wong, BCY; Fan, XM; Zhang, HB; Lin, MCM; Kung, HF; Fan, DM; Lam, SK;
Indirizzi:
Univ Hong Kong, Dept Med, Hong Kong, Hong Kong, Peoples R China Univ Hong Kong Hong Kong Hong Kong Peoples R China Kong, Peoples R China Univ Hong Kong, Inst Mol Biol, Hong Kong, Hong Kong, Peoples R China Univ Hong Kong Hong Kong Hong Kong Peoples R China Kong, Peoples R China Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Xian, Peoples R China Fourth Mil Med Univ Xian Peoples R China est Dis, Xian, Peoples R China
Titolo Testata:
CARCINOGENESIS
fascicolo: 9, volume: 22, anno: 2001,
pagine: 1393 - 1397
SICI:
0143-3334(200109)22:9<1393:NADIAI>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
NONSTEROIDAL ANTIINFLAMMATORY DRUGS; POLY(ADP-RIBOSE) POLYMERASE; CYTOCHROME-C; COLORECTAL-CANCER; EPITHELIAL-CELLS; BCL-2; ACTIVATION; INDOMETHACIN; INHIBITION; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Wong, BCY Univ Hong Kong, Dept Med, Hong Kong, Hong Kong, Peoples R China Univ Hong Kong Hong Kong Hong Kong Peoples R China les R China
Citazione:
X.M. Zhou et al., "Non-steroidal anti-inflammatory drugs induce apoptosis in gastric cancer cells through up-regulation of bax and bak", CARCINOGENE, 22(9), 2001, pp. 1393-1397

Abstract

Aspirin- and non-steroidal anti-inflammatory drug (NSAID)-induced apoptosis is one of the important mechanisms for their anti-tumour effect in gastric cancer. We aimed at determining the role of bcl-2 family proteins and caspases in the apoptotic process. Gastric cancer cell lines AGS (wild-type p53) and MKN-28 (mutant p53) were used. Cell proliferation was measured by MTT assay. Apoptosis was determined by acridine orange staining. Protein expressions were determined by western blotting. Aspirin and indomethacin inhibited cell proliferation and induced apoptosis in both cells. AGS cells weremore sensitive compared with MKN-28 cells. The pro-apoptotic proteins bax and bak were overexpressed after treatment, while the protein level of bcl-2 remained unchanged. Apoptosis was accompanied by an increase in caspase-3activity and cleavage of caspase-3 and poly(ADP-ribose) polymerase. Inhibition of caspase-3 rescued aspirin-induced apoptosis. Our results suggest that one of the major pathways which mediates the anti-tumour response of aspirin and indomethacin in gastric cancer cells is through up-regulation of bax and bak and activation of caspase-3. Bax and bak are important in the chemoprevention of gastric cancer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 16:12:35