Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
Autore:
Merjan, AJ; Kanashiro, CA; Krieger, JE; Han, SW; Paiva, ACM;
Indirizzi:
Univ Fed Sao Paulo, Dept Biofis, Escola Paulista Med, BR-04023062 Sao Paulo, Brazil Univ Fed Sao Paulo Sao Paulo Brazil BR-04023062 BC3062 Sao Paulo, Brazil Univ Sao Paulo, Fac Med, Inst Coracao, Mol Biol Lab, Sao Paulo, Brazil Univ Sao Paulo Sao Paulo Brazil oracao, Mol Biol Lab, Sao Paulo, Brazil
Titolo Testata:
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
fascicolo: 9, volume: 34, anno: 2001,
pagine: 1175 - 1183
SICI:
0100-879X(200109)34:9<1175:LEANLO>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGNAL-TRANSDUCTION; BETA(2)-ADRENERGIC RECEPTOR; AT(1A) RECEPTOR; TYPE-1 RECEPTOR; BINDING-SITES; INTERNALIZATION; PROTEIN; RESIDUES; REQUIREMENTS; TERMINUS;
Keywords:
angiotensin II; agonist-induced receptor internalization endocytosis; fluorescence-tagged receptor; nuclear angiotensin receptors; renin-angiotensin system;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Paiva, ACM Univ Fed Sao Paulo, Dept Biofis, Escola Paulista Med, Rua Botucatu 862, BR-04023062 Sao Paulo, Brazil Univ Fed Sao Paulo Rua Botucatu 862 Sao Paulo Brazil BR-04023062 BC
Citazione:
A.J. Merjan et al., "Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells", BRAZ J MED, 34(9), 2001, pp. 1175-1183

Abstract

A construct (AT1R-NF) containing a "Flag" sequence added to the N-terminusof the rat AT(1), receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensinantagonists also induced endocytosis, suggesting that a common step in thetransduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/05/20 alle ore 16:41:20