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Titolo:
Glucose-based optimization of CHO-cell perfusion cultures
Autore:
Dowd, JE; Kwok, KE; Piret, JM;
Indirizzi:
Univ British Columbia, Biotechnol Lab, Vancouver, BC V6T 1Z3, Canada Univ British Columbia Vancouver BC Canada V6T 1Z3 ver, BC V6T 1Z3, Canada Univ British Columbia, Dept Biol & Chem Engn, Vancouver, BC V6T 1Z4, Canada Univ British Columbia Vancouver BC Canada V6T 1Z4 ver, BC V6T 1Z4, Canada
Titolo Testata:
BIOTECHNOLOGY AND BIOENGINEERING
fascicolo: 2, volume: 75, anno: 2001,
pagine: 252 - 256
SICI:
0006-3592(20011020)75:2<252:GOOCPC>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOLLOW-FIBER BIOREACTORS; HYBRIDOMA CELLS; TRANSIENT RESPONSES; ANTIBODY-PRODUCTION; NUTRIENT ADDITIONS; STEP CHANGES; BATCH; METABOLISM; RETENTION; GROWTH;
Keywords:
perfusion; CHO culture; glucose utilization; control; optimization; acceptable ranges; edge of failure; t-PA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Piret, JM Univ British Columbia, Biotechnol Lab, 237-6174 Univ Blvd, Vancouver, BC V6T 1Z3, Canada Univ British Columbia 237-6174 Univ Blvd VancouverBC Canada V6T 1Z3
Citazione:
J.E. Dowd et al., "Glucose-based optimization of CHO-cell perfusion cultures", BIOTECH BIO, 75(2), 2001, pp. 252-256

Abstract

Perfusion cultures of CHO cells producing t-PA were performed using acoustic filter cell retention. A robust off-line glucose analysis and predictivecontrol protocol was developed to maintain the process within approximately 0.5 mM of the glucose set point, without the need for a more fallible on-line sensor. Glucose usage (the difference between the inlet and reactor glucose concentrations) provided an easily measured indicator of overall medium utilization for mapping acceptable ranges of operation, including the edge of failure. Earlier onset of perfusion with a ramping glucose set point (1.5 mM/d) resulted in improved growth and consistency during the perfusionculture start-up. At steady state, the t-PA concentration variability increased gradually with increasing glucose usage up to approximately 22 mM, then up to 24 mM the variability increased threefold. Peak t-PA concentrations of over 90 mg/L were obtained by controlling at a glucose usage of approximately 24 mM, but these t-PA levels were not sustainable for more than 3 days. A consistent t-PA concentration of 40 mg/L was obtained at a glucose usage of 21.5 mM. (C) 2001 John Wiley & Sons, Inc.

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Documento generato il 09/04/20 alle ore 10:45:20