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Titolo:
Effect of water activity and immobilization on fatty acid selectivity for esterification reactions mediated by lipases
Autore:
Lee, CH; Parkin, KL;
Indirizzi:
Univ Wisconsin, Dept Food Sci, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 Dept Food Sci, Madison, WI 53706 USA
Titolo Testata:
BIOTECHNOLOGY AND BIOENGINEERING
fascicolo: 2, volume: 75, anno: 2001,
pagine: 219 - 227
SICI:
0006-3592(20011020)75:2<219:EOWAAI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PSEUDOMONAS-CEPACIA LIPASE; ORGANIC MEDIA; CANDIDA-ANTARCTICA; DIFFERENT SUPPORTS; SIMILAR DEPENDENCE; CATALYTIC ACTIVITY; GLYCEROL ANALOGS; REACTION-RATES; SOLVENTS; ENZYME;
Keywords:
lipase; esterification; selectivity; fatty acid; water activity; immobilization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Parkin, KL Univ Wisconsin, Dept Food Sci, Babcock Hall,1605 Linden Dr, Madison, WI 53706 USA Univ Wisconsin Babcock Hall,1605 Linden Dr Madison WI USA 53706
Citazione:
C.H. Lee e K.L. Parkin, "Effect of water activity and immobilization on fatty acid selectivity for esterification reactions mediated by lipases", BIOTECH BIO, 75(2), 2001, pp. 219-227

Abstract

The effect of water activity (a(w)) and immobilization on fatty acid (FA) selectivity of Burkholderia (formerly Pseudomonas) cepacia, Rhizomucor miehei, Candida antarctica (type B), and Candida rugosa lipases in esterification reactions was determined. Studies were based on measuring ester formation in multicompetitive reaction mixtures containing either the homologous series of even carbon number n-chain saturated FA (C4-C18) or a series of n-chain (un)saturated FA (C18:X, where X = 0-3 double bonds) as cosubstrates with 1,3-propanediol in ter-butyl methyl ether at a(w) of 0.19, 0.69, and 0.90. Activity and FA selectively patterns were similar for free and Celite-adsorbed lipases in response to changes in a(w), although specific effects were observed for selectivity of B. cepacia and C. rugosa lipases toward C16and C4/C6 FA, respectively. Also, selectivity toward unsaturated C18:X FA as a group was modulated by changes in a(w) for three of the four lipase studied. Resin-fixed lipases from R. miehei and C. antarctica exhibited profound differences in activity and FA selectively in response to changes in a(w), relative to free and Celite-bound forms. These findings suggest that FAselectivity for lipid modification is influenced by a(w) and immobilization, but that each lipase has a characteristic response to these factors in amanner that cannot be predicted. (C) 2001 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 20:28:05