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Titolo:
Accumulation of cyclic AMP in Swiss 3T3 cells adhering to a cellulose biomaterial substratum through interaction with adenylyl cyclase
Autore:
Faucheux, N; Correze, C; Haye, B; Nagel, MD;
Indirizzi:
Univ Technol Compiegne, CNRS, UMR 6600, F-60205 Compiegne, France Univ Technol Compiegne Compiegne France F-60205 -60205 Compiegne, France Fac Pharm Chatenay Malabry, INSERM, U486, Unite Rech Transduct Hormonale &Regulat Cellulai, Malabry, France Fac Pharm Chatenay Malabry Malabry France lat Cellulai, Malabry, France UFR Sci Exactas & Nat, Biochim Lab, IFR Biomol 53, CNRS,UPRES A 6021, Reims, France UFR Sci Exactas & Nat Reims France 53, CNRS,UPRES A 6021, Reims, France
Titolo Testata:
BIOMATERIALS
fascicolo: 22, volume: 22, anno: 2001,
pagine: 2993 - 2998
SICI:
0142-9612(200111)22:22<2993:AOCAIS>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
G-PROTEIN; GEOMETRIC CONTROL; CAMP; ACTIVATION; ALPHA; RECEPTOR; CYTOSKELETON; ORGANIZATION; FIBROBLASTS; FORSKOLIN;
Keywords:
Cuprophan; cAMP-dependent aggregation; cell shape;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Nagel, MD Univ Technol Compiegne, CNRS, UMR 6600, BP 20529,Domaine Biomat Biocompatibil, F-60205 Compiegne, France Univ Technol Compiegne BP 20529,Domaine Biomat Biocompatibil Compiegne France F-60205
Citazione:
N. Faucheux et al., "Accumulation of cyclic AMP in Swiss 3T3 cells adhering to a cellulose biomaterial substratum through interaction with adenylyl cyclase", BIOMATERIAL, 22(22), 2001, pp. 2993-2998

Abstract

Controlling cell shape induced by cell-substrata interaction appears of prime importance to influence subsequent biological processes such as cell migration, proliferation, differentiation or apoptosis. Studies on Swiss 3T3 fibroblasts have recently provided evidence that cell spreading is mediatedby integrins and RhoA. Our previous studies showed that on Cuprophan, a cellulose membrane (CU) to which vitronectin adhesive protein is poorly adsorbed, Swiss 3T3 cells are rounded and undergo cAMP-dependent aggregation. Incontrast, on a polyacrylonitrile membrane (AN69) that favours the adsorption of vitronectin and fibronectin, cells spread out and contain low concentrations of cAMP. We have now examined the parts played by the three components in the cAMP pathway (receptors, G-proteins and adenylyl cyclase itself)in cAMP-dependent cell aggregation on CU. Experiments with intact cells showed no interaction between the CU and receptors, or between the CU and G-proteins. Assays on membrane preparations plus the Mn-ATP substrate, which uncouples G-proteins and adenylyl cyclase, demonstrated that activation of the cAMP pathway by CU depends primarily on the catalytic activity of the adenylyl cyclase. These investigations provide essential data for the development of biomaterials that favour cell functionality. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 22:59:43