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Titolo:
Molecular and immunological characterisation of the glucose regulated protein 78 of Leishmania donovani
Autore:
Jensen, ATR; Curtis, J; Montgomery, J; Handman, E; Theander, TG;
Indirizzi:
Univ Copenhagen, Panum Inst, Inst Med Microbiol & Immunol, Ctr Med Parasitol, DK-2200 Copenhagen, Denmark Univ Copenhagen Copenhagen Denmark DK-2200 , DK-2200 Copenhagen, Denmark Copenhagen Univ Hosp, Rigshosp, Dept Infect Dis, Copenhagen, Denmark Copenhagen Univ Hosp Copenhagen Denmark Infect Dis, Copenhagen, Denmark Copenhagen Univ Hosp, Rigshosp, Dept Clin Microbiol, Copenhagen, Denmark Copenhagen Univ Hosp Copenhagen Denmark Microbiol, Copenhagen, Denmark Walter & Eliza Hall Inst Med Res, Melbourne, Vic 3050, Australia Walter & Eliza Hall Inst Med Res Melbourne Vic Australia 3050 , Australia
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
fascicolo: 1, volume: 1549, anno: 2001,
pagine: 73 - 87
SICI:
0167-4838(20010910)1549:1<73:MAICOT>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK PROTEINS; CHAIN BINDING-PROTEIN; GENE B-PROTEIN; CUTANEOUS LEISHMANIASIS; IMMUNE-RESPONSE; VISCERAL LEISHMANIASIS; TRYPANOSOMA-CRUZI; PEPTIDE SEQUENCE; PLUS BCG; TH1 TYPE;
Keywords:
amastigote; cDNA library screening; glucose regulated protein 78; Leishmania donovani;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Jensen, ATR Univ Copenhagen, Panum Inst, Inst Med Microbiol & Immunol, CtrMed Parasitol, Bldg 24-2,Blegdamsvej 3, DK-2200 Copenhagen, Denmark Univ Copenhagen Bldg 24-2,Blegdamsvej 3 Copenhagen Denmark DK-2200
Citazione:
A.T.R. Jensen et al., "Molecular and immunological characterisation of the glucose regulated protein 78 of Leishmania donovani", BBA-PROT ST, 1549(1), 2001, pp. 73-87

Abstract

To identify novel potential Leishmania vaccine antigens., antibodies from patients with visceral leishmaniasis (VL) were used to isolate clones from a cDNA expression library of L. donovani amastigotes. Glucose Regulated Protein (GRP78), a member of the 70 kDa heat-shock protein family was identified and characterised. The GRP78 gene was localised to chromosome 15 in L. donovani, L. major, and L. mexicana by pulse-field gel electrophoresis. The Leishmania GRP78 protein contain a carboxy-terminal endoplasmic reticulum retention signal sequence (MDDL) as does the Trypanosoma cruzi GRP78. Immunofluorescence using antibodies to the recombinant DNA-derived GRP78 protein showed staining localised to reticular material throughout the cytoplasm and in the perinuclear region of promastigotes, suggesting that the protein is localised in the endoplasmic reticulum. The protective efficacy of GRP78 was assessed in mice vaccine experiments. A GRP78 DNA vaccine primed for animmune response that protected C57B1/6 and C3H/Hc mice against infection with L. major. Similarly vaccination with a recombinant form of GRP78 purified from Escherichia coli and administered with Freund's as adjuvant inducedprotective immunity in C57B1/6 mice. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 18:15:23