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Titolo:
High glucose and advanced glycation end products induce phospholipid hydrolysis and phospholipid enzyme inhibition in bovine retinal pericytes
Autore:
Assero, G; Lupo, G; Anfuso, CD; Ragusa, N; Alberghina, M;
Indirizzi:
Univ Catania, Fac Med, Dept Biochem, I-95125 Catania, Italy Univ Catania Catania Italy I-95125 Dept Biochem, I-95125 Catania, Italy
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
fascicolo: 2, volume: 1533, anno: 2001,
pagine: 128 - 140
SICI:
1388-1981(20010928)1533:2<128:HGAAGE>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED PROTEIN-KINASE; HUMAN ENDOTHELIAL-CELLS; OXIDATIVE STRESS; OXIDANT STRESS; EXPERIMENTAL GALACTOSEMIA; EICOSANOID PRODUCTION; DIABETIC-RETINOPATHY; ANTIOXIDANT ENZYMES; CAPILLARY PERICYTES; MICROVASCULAR CELLS;
Keywords:
high glucose; advanced glycation end product; pericyte; phospholipid; arachidonic acids; cytosolic phospholipase A(2);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Alberghina, M Univ Catania, Fac Med, Dept Biochem, Viale Andrea Doria 6, I-95125 Catania, Italy Univ Catania Viale Andrea Doria 6 Catania Italy I-95125 aly
Citazione:
G. Assero et al., "High glucose and advanced glycation end products induce phospholipid hydrolysis and phospholipid enzyme inhibition in bovine retinal pericytes", BBA-MOL C B, 1533(2), 2001, pp. 128-140

Abstract

In the present study, we investigated the possible role of oxidative stress and the modulation of phospholipid turnover in two related models of pericyte injury, i.e., treatment with high glucose or advanced glycation end products (AGEs). Growing microcapillary pericytes from bovine retinas in culture were incubated, for 3 weeks, with 20-50 mM glucose or 2-20 muM AGEs, and peroxidation parameters (malondialdehyde, conjugated diene, hydroperoxide, glutathione (GSH) levels and lactate dehydrogenase (LDH) release) were evaluated. Arachidonate (AA) and choline release from membrane phospholipids was determined in pericytes prelabeled with [1-C-14]arachidonate and [Me-H-3]choline, respectively, and stimulated with elevated glucose or AGEs for 30 min or 2 h. [1-C-14]arachidonate and [Me-H-3]choline incorporation into phospholipids, for 2 It and 3 h respectively, was also studied in conditioned and serum-starved cultures. Finally, lysates of treated and control cellswere assayed for cytosolic phospholipase A(2) (cPLA(2)), acyl-CoA: 1-acyl-sn-glycero-3-phosphocholine O-acyltransferase (AT), CTP:phosphocholine cytidylyltransferase (CT) and microsomal choline phosphotransferase (CPT) enzyme activities. We found that high glucose and AGEs caused neither significant production of reactive oxygen species nor cell toxicity or death, unlike other cell types. Both agents had no significant effect on the cellular ultrastructure, evaluated by light and electron microscopy, AA incorporation and release, cytosolic phospholipase A(2) (cPLA(2)) and AT activities. Oil the contrary, choline incorporation into phosphatidylcholine, CT and CPT activities were significantly reduced either by 50 mM glucose or 20 muM AGEs. Simultaneously, [Me-H-3]choline release was significantly stimulated by both agents, We conclude that prolonged treatments with high glucose or AGEs are not able to induce oxidative injury in bovine retinal capillary pericytes. Nevertheless, they do induce phospholipid hydrolysis and phospholipid enzyme activity inhibition. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 14:08:03