Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Expansion of human hepatocyte populations by a retroviral gene transfer ofsimian virus 40 large T antigen
Autore:
Kobayashi, N; Westerman, KA; Taguchi, T; Sakaguchi, M; Fujiwara, T; Urata, H; Kishimoto, N; Hayashi, N; Nakaji, S; Murakami, T; Leboulch, P; Tanaka, N;
Indirizzi:
Okayama Univ, Sch Med, Dept Surg 1, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 , Dept Surg 1, Okayama 7008558, Japan Okayama Univ, Sch Med, Dept Anat 2, Okayama 700, Japan Okayama Univ Okayama Japan 700 Sch Med, Dept Anat 2, Okayama 700, Japan Okayama Univ, Sch Med, Dept Cell Biol, Okayama 700, Japan Okayama Univ Okayama Japan 700 h Med, Dept Cell Biol, Okayama 700, Japan Okayama Univ, Sch Med, Cent Res Lab, Okayama 700, Japan Okayama Univ Okayama Japan 700 Sch Med, Cent Res Lab, Okayama 700, Japan Japan Hlth Sci Fdn, Chuo Ku, Tokyo, Japan Japan Hlth Sci Fdn Tokyo Japan apan Hlth Sci Fdn, Chuo Ku, Tokyo, Japan Harvard Univ, MIT, Div Hlth Sci & Technol, Cambridge, MA 02139 USA HarvardUniv Cambridge MA USA 02139 ci & Technol, Cambridge, MA 02139 USA Kuraray Co Ltd, Dept Med Prod, Kurashiki, Okayama, Japan Kuraray Co Ltd Kurashiki Okayama Japan d Prod, Kurashiki, Okayama, Japan Harvard Univ, Brigham & Womens Hosp, Sch Med, Dept Med,Hematol Div, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 pt Med,Hematol Div, Boston, MA 02115 USA
Titolo Testata:
ASAIO JOURNAL
fascicolo: 5, volume: 47, anno: 2001,
pagine: 481 - 485
SICI:
1058-2916(200109/10)47:5<481:EOHHPB>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE LIVER-FAILURE; CONDITIONALLY IMMORTALIZED HEPATOCYTES; TRANSPLANTATION; TUMORS; SYSTEM; CELLS; RATS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Kobayashi, N Okayama Univ, Sch Med, Dept Surg 1, 2-5-1 Shikata Cho, Okayama 7008558, Japan Okayama Univ 2-5-1 Shikata Cho Okayama Japan 7008558 , Japan
Citazione:
N. Kobayashi et al., "Expansion of human hepatocyte populations by a retroviral gene transfer ofsimian virus 40 large T antigen", ASAIO J, 47(5), 2001, pp. 481-485

Abstract

A hybrid artificial liver (HAL) could be used to treat acute liver failureor to serve as a temporary support until orthotopic liver transplantation is available. Primary human hepatocytes are ideal as a source of hepatic function in a HAL device. However, the worldwide shortage of human livers available for hepatocyte isolation severely limits this form of therapy. A possible alternative is to use a tightly regulated cell line that can be economically grown in culture to have differentiated liver function. In this work, human hepatocytes were immortalized with a retroviral vector SSR#69 expressing the genes of simian virus 40 large T antigen and herpes simplex virus-thymidine kinase. One of the resulting clones, NKNT-3, showed the gene expression of differentiated liver function and were sensitive to the antiviral agent ganciclovir. When transplanted into the spleen of rats subjected to 90% hepatectomy, NKNT-3 cells prolonged the survival of 90% hepatectomized rats. The cells provide the advantages of unlimited availability, sterility, uniformity, and freedom from pathogens. This work represents a potential novel strategy for resolving the organ shortage that currently limits theuse of primary human hepatocytes to develop a HAL.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 12:12:24