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Titolo:
Construction of a differentiated human hepatocyte cell line expressing theherpes simplex virus-thymidine kinase gene
Autore:
Kobayashi, N; Miyazaki, M; Westerman, KA; Noguchi, H; Sakaguchi, M; Totsugawa, T; Watanabe, T; Matsumura, T; Fujiwara, T; Leboulch, P; Tanaka, N; Namba, M;
Indirizzi:
Okayama Univ, Sch Med, Dept Surg 1, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 , Dept Surg 1, Okayama 7008558, Japan Okayama Univ, Sch Med, Dept Cell Biol, Okayama 700, Japan Okayama Univ Okayama Japan 700 h Med, Dept Cell Biol, Okayama 700, Japan Japan Hlth Sci Fdn, Chuo Ku, Tokyo, Japan Japan Hlth Sci Fdn Tokyo Japan apan Hlth Sci Fdn, Chuo Ku, Tokyo, Japan Harvard Univ, MIT, Div Hlth Sci & Technol, Cambridge, MA 02139 USA HarvardUniv Cambridge MA USA 02139 ci & Technol, Cambridge, MA 02139 USA Harvard Univ, Brigham & Womens Hosp, Sch Med, Dept Med,Hematol Div, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 pt Med,Hematol Div, Boston, MA 02115 USA
Titolo Testata:
ASAIO JOURNAL
fascicolo: 5, volume: 47, anno: 2001,
pagine: 476 - 480
SICI:
1058-2916(200109/10)47:5<476:COADHH>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE LIVER-FAILURE; CONDITIONALLY IMMORTALIZED HEPATOCYTES; SV40 T-ANTIGEN; TRANSPLANTATION; HEMOPERFUSION; CHARCOAL; TISSUE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Kobayashi, N Okayama Univ, Sch Med, Dept Surg 1, 2-5-1 Shikata Cho, Okayama 7008558, Japan Okayama Univ 2-5-1 Shikata Cho Okayama Japan 7008558 , Japan
Citazione:
N. Kobayashi et al., "Construction of a differentiated human hepatocyte cell line expressing theherpes simplex virus-thymidine kinase gene", ASAIO J, 47(5), 2001, pp. 476-480

Abstract

Transient support using a hybrid artificial liver (HAL) device is a promising treatment for the patients with acute liver failure. Primary human hepatocytes are an ideal source for HAL therapy; however, the number of human livers available for hepatocyte isolation is limited by competition for use in whole organ transplantation. To overcome this problem, we previously established a highly differentiated human fetal hepatocyte cell line OUMS-29. Considering the potential risk when using these genetically engineered cells in humans, additional safeguards should be added to make the cells more clinically useful. In this work, the herpes simplex virus thymidine kinase (HSVtk) gene was retrovirally introduced into OUMS-29 cells. One of the HSVtk-expressed clones, OUMS-29/thymidine kinase (TK), grew in chemically defined serum free medium and expressed the genes of albumin, asialoglycoproteinreceptor, glutamine synthetase, glutathione-S-transferase pi, and blood coagulation factor X. In vitro sensitivity of the cells to ganciclovir was evaluated. Intrasplenic transplantation of 50 X 10(6) OUMS-29/TK cells prolonged the survival of 90% hepatectomized rats compared with medium injection alone (control). In the present study, we have established highly differentiated immortalized human hepatocytes with tight regulation. The cells may be clinically useful for HAL treatment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 19:38:44