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Titolo:
LR11, a mosaic LDL receptor family member, mediates the uptake of ApoE-rich lipoproteins in vitro
Autore:
Taira, K; Bujo, H; Hirayama, S; Yamazaki, H; Kanaki, T; Takahashi, K; Ishii, I; Miida, T; Schneider, WJ; Saito, Y;
Indirizzi:
Chiba Univ, Grad Sch Med, Dept Genome Res & Clin Applicat, Chuo Ku, Chiba 2608670, Japan Chiba Univ Chiba Japan 2608670 n Applicat, Chuo Ku, Chiba 2608670, Japan Chiba Univ, Grad Sch Med, Dept Clin Cell Biol, Chuo Ku, Chiba 2608670, Japan Chiba Univ Chiba Japan 2608670 Cell Biol, Chuo Ku, Chiba 2608670, Japan Chiba Univ, Fac Pharmaceut Sci, Lab Clin Pharmacol, Chuo Ku, Chiba 2608670, Japan Chiba Univ Chiba Japan 2608670 Pharmacol, Chuo Ku, Chiba 2608670, Japan Kowa Co Ltd, Kowa Res Inst, Tokyo, Japan Kowa Co Ltd Tokyo JapanKowa Co Ltd, Kowa Res Inst, Tokyo, Japan Niigata Univ, Sch Med, Clin Lab, Niigata, Japan Niigata Univ Niigata Japan gata Univ, Sch Med, Clin Lab, Niigata, Japan Univ Vienna, Vienna, Austria Univ Vienna Vienna AustriaUniv Vienna, Vienna, Austria Bioctr, Dept Mol Genet, Vienna, Austria Bioctr Vienna AustriaBioctr, Dept Mol Genet, Vienna, Austria
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 9, volume: 21, anno: 2001,
pagine: 1501 - 1506
SICI:
1079-5642(200109)21:9<1501:LAMLRF>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
RABBIT ATHEROSCLEROTIC LESIONS; APOLIPOPROTEIN-E RECEPTOR-2; ALPHA-2-MACROGLOBULIN RECEPTOR; VLDL RECEPTOR; HUMAN BRAIN; ALPHA(2)-MACROGLOBULIN RECEPTOR; DIFFERENTIAL EXPRESSION; SCAVENGER RECEPTOR; ENDOTHELIAL-CELLS; SORTING RECEPTOR;
Keywords:
LDL receptors; atherosclerosis; smooth muscle cells; beta-VLDL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Bujo, H Chiba Univ, Grad Sch Med, Dept Genome Res & Clin Applicat, Chuo Ku, M6,1-8-1 Inohana, Chiba 2608670, Japan Chiba Univ M6,1-8-1 Inohana ChibaJapan 2608670 ba 2608670, Japan
Citazione:
K. Taira et al., "LR11, a mosaic LDL receptor family member, mediates the uptake of ApoE-rich lipoproteins in vitro", ART THROM V, 21(9), 2001, pp. 1501-1506

Abstract

Since the molecular identification of the low density lipoprotein receptor(LDLR), an ever increasing number of related proteins have been discovered. These receptors belonging to the LDLR family are thought to play key roles in lipoprotein metabolism in a variety of tissues, including the arterialwall. We have discovered that the expression of a 250-kDa mosaic LDLR-related protein, which we termed LR11 for the presence of 11 LDLR ligand-binding repeats, is markedly induced in smooth muscle cells in the hyperplastic intima of animal models used for the study of atherosclerosis. Here, we demonstrate that the human LR11, when overexpressed in hamster cells, binds andinternalizes 39-kDa receptor-associated protein (RAP), an in vitro ligand for all receptors belonging to the LDLR family. Furthermore, LR11 binds theapolipoprotein E (apoE)-rich lipoproteins, beta -very low density lipoproteins (VLDLs), with a high affinity similar to that of other members, such as the LDLR and VLDL receptor. RAP and beta -VLDL compete with each other; however, other serum lipoproteins are not able to inhibit their binding. LR11 shows specific binding of apoE-enriched HDL prepared from human cerebrospinal fluid as well as of beta -VLDL, suggesting that the apoE content of lipoproteins is most likely important for mediating the high-affinity bindingto the receptor. LR11-overexpressing cells are able to internalize and degrade the bound beta -VLDL; these cells also show increased accumulation of cholesterol esters when incubated with beta -VLDL. Incubation for 48 hours with beta -VLDL of LR11-overexpressing cells, but not of control cells, promotes the appearance of numerous intracellular lipid droplets. Taken together, LR11, a mosaic LDLR family member whose expression in smooth muscle cells is markedly induced in atheroma, has all the properties of a receptor for the endocytosis of lipoproteins, particularly for the incorporation of apoE-rich lipoproteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 09:06:29