Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Interleukin-6 gene -174G > C and -572G > C promoter polymorphisms are strong predictors of plasma - Interleukin-6 levels after coronary artery bypasssurgery
Autore:
Brull, DJ; Montgomery, HE; Sanders, J; Dhamrait, S; Luong, L; Rumley, A; Lowe, GDO; Humphries, SE;
Indirizzi:
Royal Free & Univ Coll London Med Sch, Dept Med, Div Cardiovasc Genet, London, England Royal Free & Univ Coll London Med Sch London England t, London, England Univ Glasgow, Dept Med, Glasgow, Lanark, Scotland Univ Glasgow Glasgow Lanark Scotland Dept Med, Glasgow, Lanark, Scotland
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 9, volume: 21, anno: 2001,
pagine: 1458 - 1463
SICI:
1079-5642(200109)21:9<1458:IG->CA>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; MYOCARDIAL-INFARCTION; REACTIVE PROTEIN; UNSTABLE ANGINA; EXTRACORPOREAL-CIRCULATION; FIBRINOGEN CONCENTRATION; CARDIOVASCULAR-DISEASE; CHLAMYDIA-PNEUMONIAE; HELICOBACTER-PYLORI; CARDIAC-SURGERY;
Keywords:
interleukin-6; genetic polymorphism; inflammation; coronary artery bypass surgery;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Brull, DJ Rayne Inst, Div Cardiovasc Genet, 5 Univ St, London WC1E 6JJ, England Rayne Inst 5 Univ St London England WC1E 6JJ WC1E 6JJ, England
Citazione:
D.J. Brull et al., "Interleukin-6 gene -174G > C and -572G > C promoter polymorphisms are strong predictors of plasma - Interleukin-6 levels after coronary artery bypasssurgery", ART THROM V, 21(9), 2001, pp. 1458-1463

Abstract

Interleukin-6 (IL-6) synthesized in response to diverse stimuli may play an important role in bridging the inflammatory and atherosclerotic processes. The acute-phase response after coronary artery bypass graft surgery (CABG) is associated with the induction and release of cytokines, such as IL-6. We have examined the effect of common polymorphisms in the IL-6 gene promoter (-1746>C, -572G>C, and -5976>A) on IL-6 levels after elective CABG. DNA extracted from the peripheral blood of 127 patients was amplified by polymerase chain reaction. IL-6 genotypes were resolved by gel electrophoresis after restriction enzyme digestion. Serum IL-6 was measured before surgery and in serial samples at 6, 24, 48, and 72 hours after CABG. Genotype distribution was as expected for a population in Hardy-Weinberg equilibrium for all polymorphisms. Rare allele frequencies (+/-95% CIs) were similar to thosereported previously: -597A 0.36 (0.30 to 0.42), -572C 0.07 (0.04 to 0.10),and -1740 0.37 (0.31 to 0.43). The -1746>C and -5976>A genotypes were in strong allelic association (Delta =0.97, P<0.001). Baseline IL-6 levels did not significantly differ between patients with different genotypes for any polymorphism. However, 6 hours after CABG, peak IL-6 levels were significantly higher (P=0.03) in carriers of the -572C allele than in those of the -572GG genotype (355 +/- 67 versus 216 +/- 13 pg/mL, respectively) and in those with genotype -174CC compared with -174G allele carriers (287 +/- 31 versus 227 +/- 15 pg/mL, respectively; P=0.04). These effects remained statistically significant after adjusting for possible confounders, including age,sex, smoking, duration of cardiopulmonary bypass, aortic cross-clamp time,and total duration of surgery. These data demonstrate that IL-6 promoter polymorphisms influence peak IL-6 production after CABG, suggesting that these polymorphisms, which are functional in vitro, are also functional in vivo, suggesting a genetic influence on IL-6 levels after acute severe injury.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 18:29:55