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Titolo:
Construction and characterization of BAC libraries for three fish species;rainbow trout, carp and tilapia
Autore:
Katagiri, T; Asakawa, S; Minagawa, S; Shimizu, N; Hirono, I; Aoki, T;
Indirizzi:
Tokyo Univ Fisheries, Lab Genet & Biochem, Minato Ku, Tokyo 1088477, JapanTokyo Univ Fisheries Tokyo Japan 1088477 Minato Ku, Tokyo 1088477, Japan Keio Univ, Sch Med, Dept Mol Biol, Tokyo, Japan Keio Univ Tokyo JapanKeio Univ, Sch Med, Dept Mol Biol, Tokyo, Japan
Titolo Testata:
ANIMAL GENETICS
fascicolo: 4, volume: 32, anno: 2001,
pagine: 200 - 204
SICI:
0268-9146(200108)32:4<200:CACOBL>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARTIFICIAL CHROMOSOME LIBRARY; FACTOR-BASED VECTOR; LINKAGE MAP; HUMAN DNA; GENES;
Keywords:
bacterial artificial chromosome (BAC); carp Cyprinus carpio; rainbow trout Oncorhynchus mykiss; tilapia Oreochromis niloticus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Aoki, T Tokyo Univ Fisheries, Lab Genet & Biochem, Minato Ku, Koran 4-5-7,Tokyo 1088477, Japan Tokyo Univ Fisheries Koran 4-5-7 Tokyo Japan 108847788477, Japan
Citazione:
T. Katagiri et al., "Construction and characterization of BAC libraries for three fish species;rainbow trout, carp and tilapia", ANIM GENET, 32(4), 2001, pp. 200-204

Abstract

Bacterial artificial chromosome (BAC) libraries are important tools for genomic research. We have constructed seven genomic BAC libraries from three fish species, rainbow trout (Oncorhynchus mykiss), carp (Cyprinus carpio) and tilapia (Oreochromis niloticus). The two rainbow trout BAC libraries have average insert sizes of 58 and 110 kb. The average size of inserts in thecarp BAC library is 160 kb. The average insert sizes of the four tilapia BAC libraries are 65, 105, 145 and 194 kb, respectively. These libraries represent good coverage of each genome (2-64 x coverage). The libraries can bescreened by conventional colony hybridization and provide a starting pointfor the construction of high-density filtres or polymerase chain reaction (PCR) screening approaches. These BAC libraries will facilitate the positional cloning of quantitative trait loci (QTLs) for a variety of economicallyimportant traits in these species.

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Documento generato il 08/04/20 alle ore 10:36:28