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Titolo:
Bovine melanocortin receptor 4: cDNA sequence, polymorphisms and mapping
Autore:
Haegeman, A; Coopman, F; Jacobs, K; Mattheeuws, M; Van Zeveren, A; Peelman, L;
Indirizzi:
State Univ Ghent, Fac Med Vet, Dept Anim Nutr Genet Breeding & Ethol, B-9820 Merelbeke, Belgium State Univ Ghent Merelbeke Belgium B-9820 hol, B-9820 Merelbeke, Belgium
Titolo Testata:
ANIMAL GENETICS
fascicolo: 4, volume: 32, anno: 2001,
pagine: 189 - 192
SICI:
0268-9146(200108)32:4<189:BMR4CS>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
BODY-WEIGHT REGULATION; OBESE GENE-PRODUCT; LEPTIN PATHWAYS; OB/OB MICE; RADIATION;
Keywords:
cDNA; melanocortin receptor 4; mutation; PCR; RFLP; SSCP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Peelman, L State Univ Ghent, Fac Med Vet, Dept Anim Nutr Genet Breeding & Ethol, B-9820 Merelbeke, Belgium State Univ Ghent Merelbeke Belgium B-9820 Merelbeke, Belgium
Citazione:
A. Haegeman et al., "Bovine melanocortin receptor 4: cDNA sequence, polymorphisms and mapping", ANIM GENET, 32(4), 2001, pp. 189-192

Abstract

A cDNA encoding the bovine melanocortin receptor 4 (MC4R) was cloned and sequenced. Comparing human, pig and rat homologues showed a 87, 85 and 89% identity on the DNA level, respectively, and over 90% on the protein level. The bovine MC4R gene was mapped to BTU 24 by radiation hybrid mapping. Two nucleotide changes were identified by single stranded conformation polymorphism (SSCP) and sequencing. The substitutions proved to be a T to C and G (allele B) to A (allele A) resulting, respectively, in a conservative valineto alanine substitution (Val 145 Ala) and an alanine to threonine (Ala 172Thr). Using PCR-RFLP, 13 different cattle breeds were screened for the presence of the Ala 172 Thr substitution. With the exception of one Red Pied animal, allele A could only be detected in Red Holstein animals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 07:16:09