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Titolo:
VEGF induces airway epithelial cell proliferation in human fetal lung in vitro
Autore:
Brown, KRS; England, KM; Goss, KL; Snyder, JM; Acarregui, MJ;
Indirizzi:
Univ Iowa, Coll Med, Dept Pediat, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 ed, Dept Pediat, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Dept Anat & Cell Biol, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 nat & Cell Biol, Iowa City, IA 52242 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 4, volume: 281, anno: 2001,
pagine: L1001 - L1010
SICI:
1040-0605(200110)281:4<L1001:VIAECP>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; SURFACTANT-ASSOCIATED PROTEIN; FACTOR GENE; VASCULAR ENDOTHELIUM; FACTOR RECEPTORS; MESSENGER-RNAS; FACTOR-ALPHA; IN-VITRO; EXPRESSION; ACID;
Keywords:
vascular endothelial growth factor; KDR; neuropilin-1; lung epithelium; surfactant proteins; cell proliferation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Acarregui, MJ Univ Iowa, Coll Med, Dept Pediat, 200 Hawkins Dr, Iowa City,IA 52242 USA Univ Iowa 200 Hawkins Dr Iowa City IA USA 52242 IA 52242 USA
Citazione:
K.R.S. Brown et al., "VEGF induces airway epithelial cell proliferation in human fetal lung in vitro", AM J P-LUNG, 281(4), 2001, pp. L1001-L1010

Abstract

Vascular endothelial growth factor (VEGF) is a potent endothelial cell mitogen involved in normal and abnormal angiogenesis. VEGF mRNA and protein are abundant in distal epithelium of midtrimester human fetal lung. In the present study, we identified immunoreactivity for KDR, a major VEGF-specific receptor, in distal lung epithelial cells of human fetal lung tissue, suggesting a possible autocrine or paracrine regulatory role for VEGF in pulmonary epithelial cell growth and differentiation. Addition of exogenous VEGF to human fetal lung explants resulted in increased epithelium volume densityand lumen volume density in the tissues, both morphometric parameters of tissue differentiation. Cellular proliferation demonstrated by bromodeoxyuridine uptake was prominent in distal airway epithelial cells and increased in the VEGF-treated explants. VEGF-treated explants also demonstrated increased surfactant protein (SP) A mRNA, SP-C mRNA, and SP-A protein levels compared with controls. However, SP-B mRNA levels were unaffected by VEGF treatment. [H-3]choline incorporation into total phosphatidyleholine was increased by VEGF treatment, but incorporation into disaturated phosphatidylcholine was not affected by exogenous VEGF. Based on these observations, we conclude that VEGF may be an important autocrine growth factor for distal airwayepithelial cells in the developing human lung.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 21:45:44